A1si confocal laser microscope

L3 animals were mounted in 5 mM sodium azide/M9 buffer on slides with 3% agar pad. pmk-1(re170[pmk-1::mNG^3xFlag]) animals were grown on Comamonas sp. (DA1877) (Avery and Shtonda, 2003 (link)) and mounted in 2 mg/mL tetramisole in M9 buffer on slides with 3% agar pad. We hypothesized that stress caused constitutive translocation to the nucleus. However, growth on Comamonas sp. bacteria, which are thought to be non-inflammatory (Avery and Shtonda, 2003 (link)), did not alter the degree of nuclear translocation. Similarly, mounting animals on tetramisole rather than sodium azide did not abolish nuclear translocation. All images were captured by A1si Confocal Laser Microscope (Nikon) using NIS Elements Advanced Research, Version 4.40 software (Nikon).

L1 animals were mounted onto slides with a 3% NG agar pad in 5 μL of 2 mg/mL tetramisole/M9 buffer. Confocal images were captured by A1si Confocal Laser Microscope (Nikon) with 488, 561nm lasers using NIS Elements Advanced Research, Version 4.40 software (Nikon). To ensure consistent exposures the same settings were used in imaging of both CRISPR tagged strains: 488nm (115, 0, 5), 561nm (160, 0, 10) (HV, Offset, Laser Power).