Anti mouse cd69 fitc clone h1.2f3

Manufactured by Thermo Fisher Scientific

The Anti-mouse CD69 FITC clone: H1.2F3 is a laboratory reagent used for the detection of mouse CD69 expression by flow cytometry. It is conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate) to enable the visualization and quantification of CD69-positive cells.

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Lab products found in correlation

Foxp3 apc fjk 16s, by Thermo Fisher Scientific (2 mentions) Anti cd3 apc cy7 clone 17a2, by BD (2 mentions) Pe anti mouse cd8a ly 2 53 6.7, by BD (2 mentions)

Spelling variants of this product

CD69-FITC (24 citations) Anti-CD69 (19 citations) CD69 (H1.2F3, (14 citations) CD69 (clone H1.2F3, (9 citations) Anti-CD69 (H1.2F3) (9 citations) Anti-CD69 FITC (7 citations) Anti-CD69 (clone H1.2F3, (4 citations) CD69-FITC (clone H1.2F3, (2 citations) ; clone H1.2F3 (2 citations) Anti-mouse CD69 (2 citations)

2 protocols using anti mouse cd69 fitc clone h1.2f3

T Cell Flow Cytometry Panel Protocol

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The T cell flow cytometry panel included the following directly conjugated primary antibodies: anti-mouse CD45 superbright 600 clone: 30-511 (ref# 63-0451-82, eBioscience), anti-CD3 APC-Cy7 clone 17A2(BD Biosciences, cat # 560590), eBioscience anti-mouse CD4 PE-Cy7 clone: RM4-5 (Ref # 25-0042-82, Invitrogen), PE anti-mouse CD8a (Ly-2)(53-6.7) (cat # 553032, BD), anti-mouse CD69 FITC clone: H1.2F3 (Ref# 11-0691-81, eBioscience), and Foxp3 (FJK-16s) APC (eBioscience). Gating strategies are as follows:
CD4+ T cell: live/CD45+/CD3+/CD4+/Foxp3-
CD8+ T cell: live/CD45+/CD3+/CD8+
Treg: live/CD45+/CD3+/CD4+/Foxp3+
Activated CD8+ T cell: live/CD45+/CD3+/CD8+/CD69+

Huntington K.E., Louie A.D., Srinivasan P.R., Schorl C., Lu S., Silverberg D., Newhouse D., Wu Z., Zhou L., Borden B.A., Giles F.J., Dooner M., Carneiro B.A, & El-Deiry W.S. (2023). GSK-3 Inhibitor Elraglusib Enhances Tumor-Infiltrating Immune Cell Activation in Tumor Biopsies and Synergizes with Anti-PD-L1 in a Murine Model of Colorectal Cancer. International Journal of Molecular Sciences, 24(13), 10870.

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Comprehensive T Cell Flow Cytometry Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

The T cell flow cytometry panel included the following directly-conjugated primary antibodies: Anti-mouse CD45 superbright 600 clone: 30–511 (ref# 63-0451-82, eBioscience), anti-CD3 APC-Cy7 clone 17A2(BD Biosciences, cat # 560590), eBioscience anti-mouse CD4 PE-Cy7 clone: RM4–5 (Ref # 25-0042-82, Invitrogen), PE anti-mouse CD8a (Ly-2)(53–6.7) (cat # 553032, BD), Anti-mouse CD69 FITC clone: H1.2F3 (Ref# 11-0691-81, eBioscience), and Foxp3 (FJK-16s) APC (eBioscience). Gating strategies are as follows:
CD4+ T cell: live/CD45+/CD3+/CD4+/Foxp3−
CD8+ T cell: live/CD45+/CD3+/CD8+
Treg: live/CD45+/CD3+/CD4+/Foxp3+
Activated CD8+ T cell: live/CD45+/CD3+/CD8+/CD69+

Huntington K.E., Louie A.D., Srinivasan P.R., Schorl C., Lu S., Silverberg D., Newhouse D., Wu Z., Zhou L., Borden B.A., Giles F.J., Dooner M., Carneiro B.A, & El-Deiry W.S. (2023). GSK-3 inhibitor elraglusib enhances tumor-infiltrating immune cell activation in tumor biopsies and synergizes with anti-PD-L1 in a murine model of colorectal cancer. bioRxiv.

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