Cyto-ID Green dye and MitoSox were purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). LysoTracker was purchased from Invitrogen (SanDiego, CA, USA). FITC-AnnexinV/PI kit was purchased from BD Biosciences (Franklin Lakes, NJ, USA). The mouse antibody to ASS was obtained from BD Biosciences. All the other primary antibodies including antibodies to β-actin, LC3B, cleaved caspase 3, PARP, anti-cleaved PARP, phospho-mTOR (Ser2448), phospho-Akt (Ser473), p70S6 kinase phospho (pS371), phospho-4EBP1-pT45 were obtained from Cell Signaling Technology (Danvers, MA, USA). The secondary antibodies were purchased from MR Biotech (Shanghai, China). The autophagy inhibitors of the PI3K inhibitor LY294002 and the lysosomal inhibitor CQ, 3-(4,5-dimetrylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) as well as L -arginine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Z-VAD-fmk and NAC were obtained from Beyotime Institute of Biotechnology (Haimen, Jiangsu Province, China).
3 4 5 dimetrylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt
Manufactured by Merck Group
Sourced in United States
3-(4,5-dimetrylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) is a chemical compound used as a colorimetric assay for measuring the activity of enzymes that reduce metabolic dyes, indicating the presence of viable cells. It is a yellow, water-soluble tetrazolium salt.
Automatically generated - may contain errors
Lab products found in correlation
Cyto id green dye, by Enzo Life Sciences (1 mentions)
Mitosox, by Enzo Life Sciences (1 mentions)
Z vad fmk, by Beyotime (1 mentions)
Anti cleaved parp, by Cell Signaling Technology (1 mentions)
Lysotracker, by Thermo Fisher Scientific (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
L arginine, by Merck Group (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Ly294002, by Merck Group (1 mentions)
N acetyl cysteine (nac), by Beyotime (1 mentions)
Cytochrome c, by Cell Signaling Technology (1 mentions)
Grp78, by Cell Signaling Technology (1 mentions)
Eif2α, by Cell Signaling Technology (1 mentions)
Gapdh, by Wuhan Servicebio Technology (1 mentions)
Mitosox, by Beyotime (1 mentions)
N acetylcysteine nac, by MedChemExpress (1 mentions)
Gsk2606414, by MedChemExpress (1 mentions)
Bcl 2, by Wuhan Servicebio Technology (1 mentions)
Bcl2 associated x (bax), by Wuhan Servicebio Technology (1 mentions)
P eif2α, by Cell Signaling Technology (1 mentions)
4 protocols using 3 4 5 dimetrylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt
1
Autophagy and Apoptosis Regulation
2
Fuziline Modulates Mitochondrial Dysfunction
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Fuziline (CHB171026, MF: C24H39O7N, MW: 453.58, purity: 99.3%) was obtained from Chengdu Chroma‐Biotechnology Co., Ltd. (China) and prepared by dissolving in dimethyl sulfoxide as a 50 mmol/L stock solution. 3‐(4, 5‐dimetrylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide (MTT) and ISO were purchased from Sigma‐Aldrich LLC (USA). 5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethyl‐imidacarbocyanine iodide (JC‐1), 2′,7′‐dichlorodihydrofluorescein diacetate (DCFH‐DA) and MitoSOX were obtained from Beyotime Biotechnology Co., Ltd. (China). Annexin‐V‐FLUOS Staining Kit and Tunel Kit were purchased from Roche Co., Ltd. (USA). N‐acetylcysteine (NAC) and GSK2606414 were purchased from MedChemExpress Co., Ltd. (China). Antibodies against caspase‐3, p‐PERK, PERK, p‐eIF2α, eIF2α, GRP78, Chop, ATF4 and cytochrome C were obtained from Cell Signaling Technology Co., Ltd. (USA). Bcl‐2, Bax and GAPDH were purchased from Servicebio (China).
3
Levistilide A Bioactivity Assessment
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Levistilide A (purity>98%) was purchased from Chengdu Push Biological Polytron Technologies, Inc. (Chengdu, China). Its specific molecular structure was shown in Figure 1 . 3-(4,5-Dimetrylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma Chemical (St. Louis, MO, USA). The lactate dehydrogenase (LDH) Cytotoxicity Assay Kit (C0017), Calcein-AM (C2012), Dihydroethidium (DHE, S0063), Mitochondrial membrane potential detection kit (JC-1 method, C2006) were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Liproxstatin-1 (HY-12726), ferrostatin-1 (HY-100579) and ML385 (HY-100523) were purchased from Med Chem Express (MCE, New Jersey, USA). The primary antibodies, including rabbit monoclonal anti-GPX4 (BM5231), rabbit polyclonal erythroid-2-related factor 2 (Nrf2, PB9290), and rabbit monoclonal anti-solute carrier family 7 membrane 11 (SLC7A11, BM5318), were obtained from BOSTER Biological Technology Co., Ltd. Rabbit monoclonal heme oxygenase 1 (HO-1, ab68477) was obtained from Abcam, Inc. The secondary antibody Alexa Fluor 594 anti-Rabbit IgG (8889S, CST) was obtained from Cell Signaling Technology, Inc.![]()
The molecular structure and Chemical Abstracts Service (CAS) number of levistilide A (LA).
4
Comprehensive Cell Characterization Assay
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RNA kit was from RiboBio Co., Ltd (Guangzhou, China). Trizol reagent was from Invitrogen (Rockville, Maryland, USA). Cell Lysis Buffer was from Cell Signaling Technology (Danvers, Massachusetts, USA). Matrigel was purchased from BD Biosciences (Bedford, Massachusetts, USA). Transwells for migration assay, SuperSignal Chemiluminescent HRP Substrate, were bought from Thermo Fisher scientific Inc. (Rockford, Illinois, USA). Antibodies against GAPDH, CyclinD1, pRb, p-pRb, MMP2, β-catenin were purchased from Abcam (Cambridge, Massachusetts, USA). 3-(4,5-dimetrylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was got from Sigma-Aldrich (St. Louis, Missouri, USA). Nuclear/Cytosol Fractionation Kit was from BioVision, Inc. (San Francisco, California, USA).
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