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Ammonium tetrathiomolybdate ttm

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Ammonium tetrathiomolybdate (TTM) is a chemical compound used in laboratory settings. It is a crystalline solid that serves as a source of molybdenum. TTM is commonly used as a precursor in the synthesis of other molybdenum-containing compounds or materials.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Chloroquine diphosphate salt cq, by Merck Group (1 mentions) Annexin 5 fitc propidium iodide apoptosis kit, by MultiSciences Biotech (1 mentions) Bafilomycin a1 baf a1, by Santa Cruz Biotechnology (1 mentions) Tert butylhydroquinone tbhq, by MedChemExpress (1 mentions) 7 aminoactinomycin d 7 aad, by MultiSciences Biotech (1 mentions) Fetal bovine serum (fbs), by Lonsera (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Celltiter 96 aqueous one solution cell proliferation assay mts kit, by Promega (1 mentions) Puromycin, by Solarbio (1 mentions) Mg132, by Beyotime (1 mentions) Dihydroethidium dhe, by Beyotime (1 mentions) Anti gm130, by BD (1 mentions) Copper 2 chloride, by Merck Group (1 mentions) Anti p84, by GeneTex (1 mentions) Anti β actin, by Merck Group (1 mentions) Ab97672, by Abcam (1 mentions) Trametinib, by Selleck Chemicals (1 mentions) Z devd fmk, by Selleck Chemicals (1 mentions) Abt 199, by Selleck Chemicals (1 mentions)

5 protocols using ammonium tetrathiomolybdate ttm

1

Investigating Cellular Responses to Oxidative Stress

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Ammonium tetrathiomolybdate (TTM, #323446) and chloroquine diphosphate salt (CQ, #C6628) were purchased from Sigma-Aldrich (St. Louis, MO, USA). MG132 (#S1748) and dihydroethidium (DHE, #S0063) were obtained from Beyotime (Shanghai, China). Tert-butylhydroquinone (tBHQ, #HY-100489) was purchased from MedChemExpress (Shanghai, China). Bafilomycin A1 (BafA1, #sc-201550) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Sodium arsenite (NaAsO2, #H4525) was obtained from Xiya Reagent (Shandong, China). 7-aminoactinomycin D (7-AAD, #AP104) and Annexin V FITC/ Propidium iodide (PI) apoptosis kit (#70-APCC101-100) were obtained from MultiSciences (Hangzhou, China). Dulbecco’s Modified Eagle Medium (DMEM, #C11995500BT) was purchased from Gibco (Grand Island, NY, USA). Fetal bovine serum (FBS, #S711-001S) was purchased from Lonsera (Shanghai, China). Penicillin-streptomycin (#15140122) was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Puromycin (#P8230) was purchased from Solarbio (Beijing, China). CellTiter 96® Aqueous One Solution Cell Proliferation Assay (MTS) kit (#G3581) was obtained from Promega (Madison, WI, USA).
Zhang M., Qiu H., Mao L., Wang B., Li N., Fan Y., Weng P., Hu S., Dong X., Qin X., Chen C., Zou Z., Yu C, & Zhang J. (2022). Ammonium tetrathiomolybdate triggers autophagy-dependent NRF2 activation in vascular endothelial cells. Cell Death & Disease, 13(8), 733.
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2

Synthesis and Characterization of Chemotherapeutic Agents

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All chemicals and solvents were purchased from Cell Signaling Technologies (Danvers, MA). cDDP was purchased from Teva Parenteral Medicines, Inc (Irvine, CA). Ammonium tetrathiomolybdate (TTM) was purchased from Sigma-Aldrich (St. Louis, MO).
Yamamoto T., Tsigelny I.F., Götz A.W, & Howell S.B. (2015). Cisplatin inhibits MEK1/2. Oncotarget, 6(27), 23510-23522.
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3

Antibody Generation and Characterization

Cited 2 times
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Antibodies to COMMD1 and COMMD9 were generated and reported previously (Li et al., 2015 (link); Phillips-Krawczak et al., 2015 (link)). Antibodies to ATP7A and ATP7B were generated by Cocalico Biologicals (Reamstown, PA, USA), after immunization of rabbits with a peptide spanning amino acids 1406-1500 for ATP7A and amino acids 1372-1465 for ATP7B. The following antibodies were obtained from commercial sources: anti-VPS35L (Pierce, PA5-28553, 1:1000), anti-β-actin (Sigma-Aldrich, A5441, 1:5000), anti-GM130 (BD Transduction Laboratories, 610822, 1:200), anti-P84 (Genetex, GTX70220, 1:500), anti-CCDC22 (ProteinTech Group, 16636-1-AP, 1:1000) and anti-CCDC93 (ProteinTech Group, 20861-1-AP, 1:1000). Ammonium tetrathiomolybdate (TTM) and copper chloride II were purchased from Sigma-Aldrich.
Singla A., Chen Q., Suzuki K., Song J., Fedoseienko A., Wijers M., Lopez A., Billadeau D.D., van de Sluis B, & Burstein E. (2021). Regulation of murine copper homeostasis by members of the COMMD protein family. Disease Models & Mechanisms, 14(1), dmm045963.
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4

Detailed Immunofluorescence and Immunoblotting Protocol

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NSC319726 (PubChem CID: 5,351,307) was purchased from MedChemExpress (Cat. HY-18634). Ammonium tetrathiomolybdate (TTM) was purchased from Sigma-Aldrich (Cat. 323,446). Retinoic acid was obtained from Meilunbio (Cat. 302–79-4).
The study utilized the following antibodies: rabbit anti-DDX4 (Bioword, BS72725, IF:1:200, WB:1:1000), mouse anti-SYCP3 (Abcam, ab97672, IF:1:200, WB:1:1000), rabbit anti-CYP17A1 (Bioword, MB10800, WB:1:1000), rabbit anti-ZO-1 (Bioword, BS71522, WB:1:1000), rabbit anti-Vinculin (Bioword, BS62273, WB:1:1000), rabbit anti-β-Catenin (Abclonal, A11932, WB:1:1000), mouse anti-GAPDH (Bioword, MB001, WB:1:1000), rabbit anti-DLAT (Proteintech,13,426–1-AP, 1:2000), rabbit anti-LIAS (Proteintech, 11,577–1-AP, 1:1000), rabbit anti-FDX1 (Bioword, BS71332, WB:1:1000), rabbit anti-STRA8 (OmnimAb, OM292470, IF:1:200, WB:1:1000), and rabbit anti-RDH10 (Affinity Biosciences, DF12105, IF:1:200, WB:1:1000).
Yi H., Chen T., He G., Liu L., Zhao J., Guo K., Cao Y., Sun P., Zhou X., Zhang B., Li C, & Wang H. (2024). Retinoic acid mitigates the NSC319726-induced spermatogenesis dysfunction through cuproptosis-independent mechanisms. Cell Biology and Toxicology, 40(1), 26.
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5

Combination Drug Screening Protocol

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A1210477 (ApexBio, B6011), ABT-199 (Selleck Chemicals, S8048), ABT-263 (M1637, AbMole), ABT-737 (Selleck Chemicals, S1002), Ammonium tetrathiomolybdate (TTM, Sigma-Aldrich, 323446), trametinib (Selleck Chemicals, S2673), WEHI-539 hydrochloride (ApexBio, A8634), vemurafenib (CT-P4032), and Z-DEVD-FMK (Selleck Chemicals, S7312) were purchased from indicated companies.
Kim Y.J., Tsang T., Anderson G.R., Posimo J.M, & Brady D.C. (2020). Inhibition of BCL2 family members increases the efficacy of copper chelation in BRAFV600E-driven melanoma. Cancer research, 80(7), 1387-1400.
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