For immunoblotting, samples were resolved on 4–12% Bis-Tris gels (NuPage) and the primary antibody [BR134 (48 (link)), 1:1,000] was diluted in PBS plus 0.1% Tween 20 and 5% non-fat dry milk. Histology and immunohistochemistry were carried out as described (46 (link)). Some sections (8 μm) were counterstained with haematoxylin. The primary antibodies were AT8 (Thermo-Fisher; 1:1,000 or 1:300), RD3 (Sigma-Millipore, 1:3,000) RD4 (Sigma-Millipore 1:100) and anti-4R (Cosmo Bio 1:400).
Anti 4r
Manufactured by Cosmo Bio
Sourced in United States, Germany
Anti-4R is a lab equipment product designed for specific applications. It serves a core function, but its intended use requires further details that are not provided in this request. A more comprehensive description cannot be given while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using anti 4r
1
Immunoblotting and Histology of Tau Proteins
2
Epitope-specific Tau and α-Synuclein Antibodies
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Anti-tau antibodies used in this study were as follows: T46 (epitope: 404–441; Thermo Fisher Scientific), pS396 (epitope: p-Ser-396; Calbiochem), RD3 (epitope: 209–224; Millipore), Anti-4R (epitope: 275–291; Cosmo Bio), TauC (epitope: 429–441; Cosmo Bio), AT8 (epitope: p-Ser-202 and p-Thr-205; Thermo Fisher Scientific), tau 360–380 (epitope: 360–380; Cosmo Bio), pS262/pT263 (epitope: p-Ser-262 and p-Thr-263; abcam). Polyclonal anti-α-synuclein (α-syn) 131–140 antibody was obtained from Cosmo Bio. Monoclonal anti-α-tubulin antibody (T9028), monoclonal and polyclonal anti-HA antibodies (H3663 and H6908), and polyclonal anti-FLAG antibody (F7425) were obtained from Sigma.
3
Antibodies for Tau Protein Analysis
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The following anti‐tau antibodies were used: T46 (mouse monoclonal, specific for residues 404–441; Thermo Fisher Scientific, Waltham, MA, USA); TauC (mouse monoclonal, specific for residues 429–441; Cosmobio, Tokyo, Japan); RD3 (mouse monoclonal, specific for 209–224; Millipore, Burlington, MA, USA); RD4 (mouse monoclonal, specific for 275–291; Millipore); anti‐4R (rabbit polyclonal, specific for 275–291; Cosmobio); AT8 (mouse monoclonal, specific for pS202 and pT205; Thermo Fisher Scientific); pS396 (mouse monoclonal, specific for pS396; Calbiochem, Darmstadt, Germany). Monoclonal and polyclonal anti‐HA antibodies (HA3663 and H6908) were purchased from Sigma Fine Chemicals (Shinagawa‐ku, Japan).
4
Tau Immunostaining of Cerebrum Sections
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The cerebrum was sliced in coronal sections vertical to the anterior and posterior commissure line. The BNM sections [12 ] at the level of the anterior commissure line were immunostained using the following antibodies raised against synthetic peptide corresponding to AT8: pSyn#64, pSer409/410, anti-3-repeat tau (RD3, monoclonal, 1:2000; Merck, Darmstadt, Germany), and 4-repeat tau (RD4, monoclonal, 1:50; Merck) and (anti-4R, monoclonal, 1:500; Cosmobio). They were further examined with Gallyas Braak silver staining [7 (link)].
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