1260 system

Manufactured by YMC

The 1260 system is a piece of laboratory equipment designed for analytical applications. It provides core functionality for various analysis tasks without interpretation or extrapolation.

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Lab products found in correlation

Ods a column, by YMC (1 mentions) C18 column, by YMC (1 mentions)

Spelling variants of this product

1260 HPLC system (3 citations) 1260 Infinity II system (2 citations)

2 protocols using 1260 system

Quantitative HPLC Analysis of Compounds

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HPLC analyses were carried out on an Agilent 1260 system equipped with a YMC-Pack ODS-A column (250 × 4.6 mm, i.d., 5 μm) at 35 °C. The gradient elution mode with acetonitrile-water containing 2% acetic acid as follows: Acetonitrile: 0–10 min, 6%–6%; 10–20 min, 6%–10%; 20–45 min, 10%–20%; 45–80 min, 20%–35%. The effluent was monitored at 254 nm, and the flow rate was 1.0 mL/min.

Sun S.W., Wang R.R., Sun X.Y., Fan J.H., Qi H., Liu Y., Qin G.Q, & Wang W. (2020). Identification of Transient Receptor Potential Vanilloid 3 Antagonists from Achillea alpina L. and Separation by Liquid-Liquid-Refining Extraction and High-Speed Counter-Current Chromatography. Molecules, 25(9), 2025.

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Analytical Determination of Riboflavin

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Cell growth was monitored by measuring optical density at 600 nm (OD₆₀₀) with UV–Vis spectrophotometer. For riboflavin measurement, samples were first diluted with 0.05 M NaOH and centrifuged at 16 000 × g for 2 min to remove the cells, the samples were then subjected to HPLC analysis using a Agilent 1260 system equipped with a YMC polymer C18 Column (4.6 × 250 mm) and riboflavin was detected by UV 370 nm (Wang et al., 2019). Separation was performed at the following conditions: 60% H₂O, 10% methanol, 20% acetonitrile and 10% phosphoric acid (2 mM) with a constant flow rate of 1 ml min⁻¹.

Yang Z., Sun Q., Tan G., Zhang Q., Wang Z., Li C., Qi F., Wang W., Zhang L, & Li Z. (2020). Engineering thermophilic Geobacillus thermoglucosidasius for riboflavin production. Microbial Biotechnology, 14(2), 363-373.

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