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5 protocols using sodium tripolyphosphate

1

Chitosan-Based Nanoparticle Delivery System

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Maraviroc was purchased from Shanghai Yudiao Chemistry Technology Co Ltd. (Shanghai, China), Tenofovir and Sodium tripolyphosphate were supplied by Macklin Biochemical Co. Ltd. (Shanghai, China), Chitosan (Molecular weight of 100–180 kDa and 70–85% deacetylated) was supplied by Dideu Medichem Ltd. (China), MRS Agar was procured from HiMedia Laboratory Pvt. Ltd. (Mumbai, India) Milli Q water (Milli Q water purification system, Merck, Germany) was used for all the experiments. All other chemicals were of analytical grade and used as obtained from the suppliers.
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2

Poloxamer and Chitosan Nanoparticles for Antiretroviral Delivery

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Poloxamer 407 (Pluronic F127), poloxamer 188 (Pluronic F68), and chitosan were supplied by Dideu Medichem Ltd. (China). Maraviroc was purchased from Shanghai Yudiao Chemistry Technology Co Ltd. (Shanghai, China). Tenofovir was supplied by Macklin Biochemical Co. Ltd. (Shanghai, China). Sodium tripolyphosphate, Span 60, and Hydroxy propyl methyl cellulose were procured from Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China). Soya bean oil was purchased from Sunola Foods Ltd. (Lagos, Nigeria).
Milli-Q water (Milli-Q water purification system, Merck, Germany) was used for all the experiments. All other chemicals were of analytical grade and used as obtained from the suppliers, without further modification.
Cell lines: HeLa cells and fetal bovine serum were purchased from Cell Lines Service GmbH, Germany. HIV-1 BaL and TZM-bl cells were obtained from Fisher Bioservices /NIH-ARP German town, MD, USA.
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Chitosan-Alginate Encapsulation of Ganoderma Peptides

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Chitosan (CS, degree of deacetylation > 85%) was provided by Shandong Haidebei Biotechnology Co., Ltd. (Shandong, China). Sodium alginate (SA, viscosity: 7000–10,000 mPa·s) and sodium tripolyphosphate (STPP) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Ganoderma lucidum peptides (GLP, 98 wt%) were obtained from Xi’an Lander Biotech Co., Ltd. (Xi’an, China). Pepsin, pig bile powder, and trypsin were supplied by Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). An ABTS radical scavenging activity assay kit was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Shanghai, China). The simulated gastric fluid (SGF, pH 1.2) was prepared by dissolving 0.6 g NaHCO3, 3.1 g NaCl, 1.1 g KCl, and 1 g Pepsin in 1 L deionized water. The simulated intestinal fluid (SIF, pH 7.4) was composed by dissolving 2.7 g NaCl, 0.31 g KCl, 10 g trypsin, and 20 g pig bile powder in 1 L deionized water. Calcium chloride and other reagents were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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Chitosan-TPP Nanoparticle Synthesis

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Chitosan (85% DD, 90% DD, 95% DD, density of CS is higher than 0.6 g/mL) and sodium tripolyphosphate (TPP) were purchased from Macklin (Shanghai, China). D-mannose (Man) were purchased from Solarbio (Beijing, China). Bovine serum albumin (BSA) was purchased from Macgene (Beijing, China). SDS-PAGE Gel Preparation Kit and protein loading buffer (5×) were purchased from Beyotime Biotechnology (Shanghai, China). MTT Cell Proliferation and Cytotoxicity Assay Kits, BSA-FITC, Hoechst 33,258 and Coomassie blue were purchased from Solarbio (Beijing, China). All other reagents used in the study were purchased from Sinopharm Chemical Reagent (Shanghai, China) with analytical grade.
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5

Chitosan-based Nanoparticle Delivery System

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Quercetin and coumarin-6 were purchased from Sigma-Aldrich (St. Louis, MO, United States). Chitosan (deacetylation degree ≥ 95%, viscosity 100–200 mpa.s, biotechnology level) and sodium tripolyphosphate (TPP) were purchased from Macklin (Shanghai, China). 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) and dimethyl sulfoxide (DMSO) were purchased from Sangon Biotech (Shanghai, China). Wheat Germ Agglutinin Alexa Fluor 594 conjugate (WGA-594) were purchased from Thermo Fisher Scientific (Eugene, OR, United States). All other chemicals and reagents of the highest quality were commercially available and used as received. Antibodies against p-IkB-α, NF-κB, COX-2, GAPDH, β-actin and lamin A/C were purchased from Cell Signaling Technology.
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