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2 protocols using slide a lyzer dialysis kit 3.5 kda mwco

1

PEG-Collagen Hydrogel Characterization

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Unless otherwise noted, all chemicals and solvents were of analytical grade and used as advised by the manufacturer. 4 and 8-arm PEG succinimidyl glutarate (PEG) was purchased from JenKem Technology (Texas, USA). Collagenase, dimethyl sulfoxide (DMSO), fluorescamine, sodium hydroxide solution (1.0 N), bovine serum albumin (BSA), fibronectin, cholera subunit A, insulin, Triton-X, and Cell Counting kit-8 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS) pH 7.4, 10X PBS, Slide-A-Lyzer dialysis kit (3.5 kDa MWCO), collagen I bovine protein solution (5 mg/mL), Collagenase, Dulbecco’s modified eagle medium nutrient mixture F-12 (DMEM/F-12) with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), insulin-transferrinselenium (ITS), Dulbecco’s phosphate-buffered saline (DPBS), antibiotic-antimycotic, Live/Dead viability/cytotoxicity staining kit, and paraformaldehyde (PFA) were purchased from Thermo Fisher Scientific (Massachusetts, USA).
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2

Synthetic PEG-based Hydrogel for Cell Culture

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Unless otherwise noted, all chemicals and solvents were of analytical grade and used as advised by the manufacturer. Four-arm PEG succinimidyl glutarate was purchased from JenKem Technology (Texas, USA). Collagenase, dimethyl sulfoxide, fluorescamine, sodium hydroxide solution (1.0 N), bovine serum albumin, fibronectin, cholera subunit A, insulin, Triton-X, and Cell Counting Kit-8 reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS; 10 × ; pH 7.4), Slide-A-Lyzer Dialysis Kit (3.5 kDa MWCO), collagen I bovine protein solution (5 mg/mL), Collagenase, Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, insulin-transferrin-selenium, Dulbecco’s PBS, antibiotic–antimycotic, Live/Dead viability/cytotoxicity staining kit, and paraformaldehyde (PFA) were purchased from Thermo Fisher Scientific (Massachusetts, USA).
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