12 mm coverslip

Manufactured by Electron Microscopy Sciences

Sourced in United States

12 mm coverslips are small circular glass or plastic sheets used in microscopy to cover and protect samples on microscope slides. They provide a flat, transparent surface for imaging and analysis.

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Lab products found in correlation

Pen strep, by Thermo Fisher Scientific (2 mentions) Dnase, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Trypsin, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Hepes, by Merck Group (2 mentions) Neurobasal, by Thermo Fisher Scientific (2 mentions) Laminin, by Thermo Fisher Scientific (2 mentions) Nahco3, by Merck Group (2 mentions) P0 p1 c57bl 6j pups, by Jackson ImmunoResearch (2 mentions) Hbss solution, by Merck Group (2 mentions) Cytosine 1 β d arabinofuranoside ara c, by Merck Group (2 mentions) Poly ornithine, by Merck Group (2 mentions) Collagen type 1, by Thermo Fisher Scientific (1 mentions) Ab9774, by Abcam (1 mentions)

4 protocols using 12 mm coverslip

Isolation and Characterization of Liver Sinusoidal Endothelial Cells

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According to a previous method [46 (link)], collagenase perfusion and percoll layers of different density gradients (25% and 50%) (GE Healthcare, MA, USA) were used to isolate LSECs. After harvested, they were seeded on 12 mm coverslips (Electron Microscopy Sciences, PA, USA) coated with type I collagen (Thermo Fisher Scientific, MA, USA) at a density of 2.5×10⁵ cells/cm². The purity of the cultured LSECs was > 90%, as determined by immunofluorescence assays with anti-von Willebrand factor (vWF) (11778-1-AP, Proteintech) and anti-endothelial cell antigen-1 (RECA-1) antibodies (ab9774, Abcam, UK) (Supplementary Figure 2).

Zhong Y., Xu M., Hu J., Huang X., Lin N, & Deng M. (2021). Inhibiting Th1/2 cells influences hepatic capillarization by adjusting sinusoidal endothelial fenestrae through Rho-ROCK-myosin pathway. Aging (Albany NY), 13(4), 5069-5086.

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Infection of Cells with A. phagocytophilum

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Neutrophils and HL-60 cells were infected with A. phagocytophilum DC organisms released from infected HL-60 cells by sonication as previously described (Troese and Carlyon, 2009 (link)). RF/6A or HEK-293T cells were infected with A. phagocytophilum DC bacteria that had been naturally released from infected RF/6A cells into the culture media as follows. Adherent host cells to be infected were seeded onto 12 mm coverslips (Electron Microscopy Sciences) and overlain with 200 µl of A. phagocytophilum-laden media from heavily infected RF/6A cells. The supernatant was spun down on top of the cells at 1000 × g for 3 min followed by a 1 h incubation at 37°C with 5% CO₂. The cells were washed with 1× PBS to remove unbound bacteria, and fresh media was added. The cells were returned to 37°C with 5% CO₂ for 24 or 48 h or specific time points, after which the cells were fixed in 4% paraformaldehyde and examined by immunofluorescence microscopy.

Truchan H.K., VieBrock L., Cockburn C.L., Ojogun N., Griffin B.P., Wijesinghe D.S., Chalfant C.E, & Carlyon J.A. (2015). Anaplasma phagocytophilum Rab10-dependent parasitism of the trans-Golgi network is critical for completion of the infection cycle. Cellular microbiology, 18(2), 260-281.

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Murine Hippocampal Primary Culture Protocol

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Hippocampal cultures were made using previously established methods (31 ). Briefly, brains from P0-P1 C57Bl/6J pups (The Jackson Laboratory, Bar Harbor, ME) were isolated, and the hippocampus was dissected in ice-cold HBSS solution (Sigma, St. Louis, MO) supplemented with 20% FBS and NaHCO₃ (4.2mM), HEPES (1mM; Sigma), pH 7.4. Dissected hippocampi were digested for 10 min with 0.25% Trypsin (Thermo Fisher Scientific), then washed and dissociated using fire polished Pasteur pipettes of decreasing diameter in ice cold HBSS containing DNase (1500 U; Sigma). The cells were pelleted, resuspended in plating media and plated at a density of 4–5×10⁵ cells/12-mm coverslip (Electron Microscopy Sciences, Hatfield, PA) coated with poly-Ornithine (0.1mg/ml; Sigma; Cat. #4638) and laminin (5μg/ml; Thermo Fisher Scientific). Cells were allowed to adhere for 15 min before addition of 0.5ml of plating media containing Neurobasal supplemented with 1X B27, 2mM Glutamax, 0.5mg/ml Pen/Strep and 5% FBS (all from Thermo Fisher Scientific) for the first 24 h. Half of the media was removed and replaced with serum-free media after 24 h. Half of the media was removed and replaced after 48 h supplemented with 4μM cytosine 1-β-d-arabinofuranoside (Ara-C; Sigma). Neurons were fed by replacing half the volume of spent media with fresh media without serum or Ara-C every week thereafter.

Bartley A.F., Fischer M., Bagley M.E., Barnes J.A., Burdette M.K., Cannon K.E., Bolding M.S., Foulger S.H., McMahon L.L., Weick J.P, & Dobrunz L.E. (2021). Feasibility of cerium-doped LSO particles as a scintillator for X-ray induced optogenetics.. Journal of neural engineering, 18(4), 10.1088/1741-2552/abef89.

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Hippocampal Neuron Culture from Neonatal Mice

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Hippocampal cultures were made using previously established methods (Chander et al., 2019) . Briefly, brains from P0-P1 C57Bl/6J pups (The Jackson Laboratory, Bar Harbor, ME) were isolated, and the hippocampus was dissected in ice-cold HBSS solution (Sigma, St. Louis, MO) supplemented with 20% FBS and NaHCO3 (4.2mM), HEPES (1mM; Sigma), pH 7.4. Dissected hippocampi were digested for 10 min with 0.25% Trypsin (Thermo Fisher Scientific), then washed and dissociated using fire polished Pasteur pipettes of decreasing diameter in ice cold HBSS containing DNase (1500 U; Sigma). The cells were pelleted, resuspended in plating media and plated at a density of 4-5×105 cells/12-mm coverslip (Electron Microscopy Sciences, Hatfield, PA) coated with poly-Ornithine (0.1mg/ml; Sigma; Cat. #4638) and laminin (5μg/ml; Thermo Fisher Scientific). Cells were allowed to adhere for 15 min before addition of 0.5ml of plating media containing Neurobasal supplemented with 1X B27, 2mM Glutamax, 0.5mg/ml Pen/Strep and 5% FBS (all from Thermo Fisher Scientific) for the first 24h. Half of the media was removed and replaced with serum-free media after 24h. Half of the media was removed and replaced after 48h supplemented and with 4µM cytosine 1-β-d-arabinofuranoside (Ara-C; Sigma). Neurons were fed by replacing half the volume of spent media with fresh media without serum or Ara-C every week thereafter.

Bartley A.F., Fischer M., Bagley M.E., Barnes J., Burdette M.K., Cannon K.E., Bolding M., Foulger S.H., McMahon L.L., Weick J.P., & Dobrunz L.E. (2020). X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2.

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