Agilent 7890a gas

Manufactured by Agilent Technologies

Sourced in Canada

The Agilent 7890A gas chromatograph is a laboratory instrument designed for the separation, identification, and quantification of chemical compounds in complex mixtures. It operates by using a carrier gas, such as helium or nitrogen, to transport the sample through a chromatographic column, where the different compounds are separated based on their respective boiling points and interactions with the column's stationary phase. The 7890A model offers advanced features and capabilities to meet the needs of a wide range of analytical applications.

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Lab products found in correlation

Db 5ms column, by Agilent Technologies (1 mentions) Zirconia silica bead, by Biospec (1 mentions) Butyric, by Merck Group (1 mentions) Isovaleric, by Merck Group (1 mentions) Valeric, by Merck Group (1 mentions) 7683b auto injector, by Agilent Technologies (1 mentions) Lactic acid, by Merck Group (1 mentions)

Close spelling variants of this product

Agilent 7890A gas chromatography Agilent 7890A-5975C gas chromatography-mass spectrometry Agilent Technology 7890A-Gas Chromatograph system Agilent 7890A gas chromatography system Agilent 7890A gas chromatograph Agilent 7890A series gas chromatograph 7890A gas Agilent 7890A

4 protocols using agilent 7890a gas

GC-MS Analysis of Sugars

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Agilent 7890A gas chromatograph coupled to a 5975C mass spectrometer and a DB-5MS column (30 m length × 0.25 mm i.d. × 0.25 μm film thickness, J&W Scientific, USA) was employed for GC–MS analysis of sugars. Helium was used as carrier gas at a flow rate of 1 mL/min. The volume of injection was 1 μL and the split ratio was 10:1. The oven temperature was held at 70 °C for 4 min, and then raised to 310 at 5 °C/min and held at the temperature for 10 min. All samples were analyzed in both full scan (mass range of 40–510 amu) and selective ion scan mode. The injector inlet and transfer line temperature were 290 and 280 °C, respectively.

Sun S., Wang H., Xie J, & Su Y. (2016). Simultaneous determination of rhamnose, xylitol, arabitol, fructose, glucose, inositol, sucrose, maltose in jujube (Zizyphus jujube Mill.) extract: comparison of HPLC–ELSD, LC–ESI–MS/MS and GC–MS. Chemistry Central Journal, 10, 25.

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Quantification of Trehalose by GC-MS

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The GC–MS (gas chromatography–mass spectrometry) technique was to quantify trehalose levels in both cells and tissues. The isotope [¹³C] trehalose (Omicron Biochemicals, TRE-002) was used as an internal standard during the sample preparation. Samples were extracted into isopropanol:acetonitrile:water (3:3:2), centrifuged at 18,000g (15 min) and dried under N₂ gas. N-Methyl-N-(trimethysilyl) trifluoroacetamide with 10% pyridine in CH₃CN was then used to derivatize samples for analysis by GC–MS using Agilent 7890A gas chromatograph interfaced to Agilent 5975C mass spectrometer and HP-5ms gas chromatography column (30 m per 0.25-mm internal diameter per 0.25-μm film coating). A temperature gradient was used starting at 80°C for 2 min, linearly increased at 10°C min⁻¹, ending at 300°C for an additional 2 min. Samples were subjected to electron ionization mode using source temperature (200 °C), electron energy (70 eV), emission current (300 μA) and injector/transfer line temperatures (250 °C). Monitored ions for trehalose and [¹³C] trehalose were m/z 361 and m/z 367, respectively.

Sergin I., Evans T.D., Zhang X., Bhattacharya S., Stokes C.J., Song E., Ali S., Dehestani B., Holloway K.B., Micevych P.S., Javaheri A., Crowley J.R., Ballabio A., Schilling J.D., Epelman S., Weihl C.C., Diwan A., Fan D., Zayed M.A, & Razani B. (2017). Exploiting macrophage autophagy-lysosomal biogenesis as a therapy for atherosclerosis. Nature Communications, 8, 15750.

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Quantifying SCFAs and BCAAs in Fecal Samples

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SCFAs and BCAAs were quantified with an Agilent 7890A gas chromatograph coupled with an Agilent 5975 mass spectrometer (Agilent Technologies Santa Clara, CA) using a propyl esterification method as previously described^{44 ,45}. Approximately 25 mg fresh human fecal samples were mixed with 1 mL of 0.005 M NaOH (containing 10 μg/mL internal standard caproic acid-6,6,6-d3), homogenized thoroughly with 1.0 mm diameter zirconia/silica beads (BioSpec, Bartlesville, OK) and centrifuged (13,200g, 4 °C, 20 min). 500 μL of supernatant was collected and mixed with an aliquot of 500 μL of 1-propanol/pyridine (v/v=3/2), then 100 μL of propyl chloroformate was added subsequently following a brief vortex for 1 minute. Samples were incubated at 60 °C for 1 hour. The derivatized samples were extracted with a two-step hexane extraction as described⁴⁵. 300 μL of hexane was added to samples, following centrifuge (2 000g, 4 °C, 5 min) and 300 μL upper layer was transferred to an autosampler vial. Another 200 μL of hexane was added to samples and the additional 200 μL upper layer was transferred and combined with the first extraction. A total 500 μL volume of extracts were obtained for GC-MS analysis. A standard curve was drafted for each analyte to quantify biological concentration in human fecal samples.

von Schwartzenberg R.J., Bisanz J.E., Lyalina S., Spanogiannopoulos P., Ang Q.Y., Cai J., Dickmann S., Friedrich M., Liu S.Y., Collins S.L., Ingebrigtsen D., Miller S., Turnbaugh J.A., Patterson A.D., Pollard K.S., Mai K., Spranger J, & Turnbaugh P.J. (2021). Caloric restriction disrupts the microbiota and colonization resistance. Nature, 595(7866), 272-277.

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Quantification of Short-Chain Fatty Acids in Cecal Content

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Samples of cecal contents preserved in 0.1% trifluoroacetic acid (1:1, vol/vol) and immediately frozen in liquid nitrogen and stored at -80°C were used for the analysis of SCFA. The analysis was performed using an Agilent 7890A gas chromatography system coupled with a flame ionization detector and a 7683B autoinjector (Agilent Technologies, Santa Clara, CA). The SCFA were separated using an SGE BP21 capillary column (30 m × 0.53 mm; film thickness = 0.5; SGE Analytical Science, Melbourne, Australia). Helium was supplied as the carrier gas at flow rate of 5 mL/min. The initial oven temperature was 85°C, raised to 180°C at 6°C/min, and held for 5 min. The temperature of the flame ionization detector and injection port were 290 and 250°C, respectively. Samples (0.5 μL of clear supernatant) were injected in a split mode with a ratio of 10:1. The content of the individual compounds was measured using external standards of acetic, propionic, butyric, valeric, isovaleric, and lactic acid (Sigma-Aldrich, Saint Louis, MO).

Wasilewska E., Zlotkowska D, & Wroblewska B. (2019). Yogurt starter cultures of Streptococcus thermophilus and Lactobacillus bulgaricus ameliorate symptoms and modulate the immune response in a mouse model of dextran sulfate sodium-induced colitis. Journal of dairy science, 102(1).

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