Rabbit anti psmad2 3

Manufactured by Abcam

Sourced in United States

Rabbit anti-pSmad2/3 is a primary antibody that specifically recognizes the phosphorylated forms of SMAD2 and SMAD3 proteins. These proteins are key mediators of the TGF-beta signaling pathway.

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Lab products found in correlation

Rabbit anti sox2, by Abcam (1 mentions) Rabbit anti tgf β1, by Abcam (1 mentions) Rabbit anti e cadherin, by Abcam (1 mentions) Rabbit anti oct4, by Abcam (1 mentions) Rabbit anti snail, by Abcam (1 mentions) Mouse anti β actin, by ZSGB-BIO (1 mentions) Enhanced chemiluminescence kit, by Cytiva (1 mentions) Rabbit anti gapdh, by Cell Signaling Technology (1 mentions) Rabbit anti osterix, by Abcam (1 mentions)

Close spelling variants of this product

Rabbit anti- (5 citations)

2 protocols using rabbit anti psmad2 3

Pluripotency and Epithelial-Mesenchymal Transition Markers

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Primary antibodies used in this study included rabbit anti-SOX-2 (1:1000 dilution, Abcam, Cambridge, UK), rabbit anti-Nanog (1:1000 dilution, CST, Danvers, Massachusetts, USA), rabbit anti-OCT-4 (1:1000 dilution, Abcam), rabbit anti-pSmad2/3 (1:500 dilution, Abcam), rabbit anti-Snail (1:500 dilution, Abcam), rabbit anti-E-cadherin (1:500 dilution, Abcam), rabbit anti-TGF-β1(1:1000 dilution, Abcam), rabbit anti-N-cadherin (1:1000 dilution, Wanleibio), rabbit anti-Slug (1:1000 dilution, Wanleibio) and mouse anti-β-actin (1:1000 dilution, ZSGB-BIO, Beijing, China).

Qi Y., Dong S.S., He Y.L., Liu Z.H., Huang Y.L., Wang N., Zhang Z., Li Z., Shi M.E., Feng X., Yao Q., Zou H., Hu J.M., Pang L.J, & Li F. (2022). SYT-SSX1 enhances the invasiveness and maintains stem-like cell properties in synovial sarcoma via induction of TGF-β1/Smad signaling. BMC Cancer, 22, 166.

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Subchondral Bone Marrow Western Blot

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Western blotting was performed on the tibial subchondral bone marrow. The samples were separated by SDS-PAGE gel and transferred onto a nitrocellulose membrane. After incubation with specific primary and secondary antibodies, signal was detected by an enhanced chemiluminescence kit (Amersham Biosciences). The primary antibodies used were as follows: rabbit anti-Osterix (Abcam), rabbit anti-pSMAD 2/3 (Abcam) and rabbit anti-GAPDH (1:1,000, 5174, Cell Signaling Technology). The experiments were repeated three times and a representative film was selected.

Zhu J., Zhu Y., Xiao W., Hu Y, & Li Y. (2020). Instability and excessive mechanical loading mediate subchondral bone changes to induce osteoarthritis. Annals of Translational Medicine, 8(6), 350.

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