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Ab11213

Manufactured by Abcam
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Ab11213 is a primary antibody designed for use in various immunoassay applications. This product is manufactured and quality-controlled by Abcam.

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Lab products found in correlation

Phospho histone h3, by Merck Group (1 mentions) Caspase 3, by Promega (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) A21121, by Thermo Fisher Scientific (1 mentions)

2 protocols using ab11213

1

Immunocytochemistry Validation of Implanted RPE Cells

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Some paraffin sections obtained from implant regions were processed for immunocytochemistry against pan-cytokeratin (1:100; catalog number ab11213, Abcam), ezrin (1:50; CST), MCT-1 (1:50; LifeSpan Biosciences), Ki67 (1:50; Sigma), phosphohistone H3 (1:50; Millipore), caspase-3 (1:50, Promega), and human-specific antibody SC121 (1:50; Stem Cells) to verify the presence of human RPE cells on the subretinally implanted cell carriers. Pan-cytokeratin was visualized with the Biotin-ExtrAvidin-AEC chromogen system. All other primary antibodies were visualized using Alexa Fluor secondary antibodies as mentioned above.
Stanzel B.V., Liu Z., Somboonthanakij S., Wongsawad W., Brinken R., Eter N., Corneo B., Holz F.G., Temple S., Stern J.H, & Blenkinsop T.A. (2014). Human RPE Stem Cells Grown into Polarized RPE Monolayers on a Polyester Matrix Are Maintained after Grafting into Rabbit Subretinal Space. Stem Cell Reports, 2(1), 64-77.
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2

Immunostaining and Imaging Analysis of Kidney Progenitor Cells

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The following primary antibodies were used: mouse anti-calbindin D28K
(Sigma-Aldrich C9848), mouse anti-cytokeratin (Abcam Ab11213 and Ab115959),
rabbit anti-SIX2 (Proteintech 11562–1-AP), rabbit anti-JAG1 (Abcam
ab7771), mouse anti-SIX2 (Abnova H00010736-MO1), rat anti-Ecad (Invitrogen
13–1900). The mouse SIX2 primary was used in conjunction with an
isotype-specific secondary antibody (Life technologies A21121) to reduce
background staining. Alexa Fluor-conjugated secondary antibodies (Life
Technologies) were used to detect primary antibodies and DAPI (Sigma-Aldrich
D8417) was used at 1:2000 to label nuclei. Whole mount immunofluorescence,
confocal microscopy, and optical projection tomography was carried out according
to published protocols 22 (link).
Cell counts per niche (confocal) and niche counts (OPT) were performed as
reported 22 (link). Quantitative
analysis of SIX2 fluorescence intensity is detailed in supplementary methods.
Combes A.N., Wilson S., Phipson B., Binnie B.B., Ju A., Lawlor K.T., Cebrian C., Walton S.L., Smyth I.M., Moritz K.M., Kopan R., Oshlack A, & Little M.H. (2017). Haploinsufficiency for SIX2 increases nephron progenitor proliferation leading to elevated branching and nephron number.. Kidney international, 93(3), 589-598.
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