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Isopropyl 1 thio β d galactopyranoside iptg

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Isopropyl 1-thio-β-D-galactopyranoside (IPTG) is a synthetic chemical compound commonly used as an inducer in bacterial cell cultures. It is a structural analog of allolactose, a natural inducer of the lac operon in Escherichia coli. IPTG is used to activate the expression of recombinant proteins in bacterial expression systems.

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Lab products found in correlation

Yeast extract, by BD (3 mentions) Tryptone, by BD (3 mentions) Sodium chloride (nacl), by Avantor (1 mentions) P 10 desalting column, by GE Healthcare (1 mentions) Gdp glucose, by Merck Group (1 mentions) Udp galactose, by Merck Group (1 mentions) Triton x 100, by Thermo Fisher Scientific (1 mentions) Udp glucose, by Merck Group (1 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Avantor (1 mentions) Udp n acetylglucosamine, by Merck Group (1 mentions) Udp glucuronic acid, by Merck Group (1 mentions) Adp glucose, by Merck Group (1 mentions) Fe nh4 2 so4 2, by Merck Group (1 mentions) Fecl3, by Merck Group (1 mentions)

3 protocols using isopropyl 1 thio β d galactopyranoside iptg

1

Glycosyltransferase Activity Assay

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Reagent grade chloroform, methanol, and silica
gel 60 thin-layer chromatography (TLC) plates (layer thickness of
150–200
μm) were obtained from EMD Chemicals Inc. (Gibbstown,
NJ). [32P]Pi, UDP-[U-14C]glucose,
UDP-[U-14C]galactose, and GDP-[U-14C]mannose
were from
PerkinElmer Life and Analytical
Sciences, Inc. (Waltham, MA). Yeast extract, agar, and tryptone were
from BD Biosciences. Sodium chloride and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES) were from VWR International (West Chester, PA). Triton
X-100 was from Thermo Fisher Scientific (Waltham, MA). Isopropyl 1-thio-β-d-galactopyranoside (IPTG) was from Invitrogen Corp. (Carlsbad,
CA). UDP-glucose, UDP-galactose, UDP-glucuronic acid, UDP-galacuronic
acid, ADP-glucose, GDP-glucose, UDP-N-acetylglucosamine,
and ADP-mannose were purchased from Sigma-Aldrich.
The P-10 desalting column was from GE. All other chemicals were reagent
grade and were purchased from either Sigma-Aldrich (St. Louis, MO)
or Mallinckrodt Baker, Inc. (Phillipsburg, NJ).
Qian J., Garrett T.A, & Raetz C.R. (2014). In Vitro Assembly of the Outer Core of the Lipopolysaccharide from Escherichia coli K-12 and Salmonella typhimurium. Biochemistry, 53(8), 1250-1262.
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2

Thin Layer Chromatography of Lipids

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Chloroform, methanol, and silica gel 60 (0.25 mm) thin layer chromatograph (TLC) plates and high-performance analytical TLC (HPTLC) plates were from EMD Chemicals (Gibbstown, NJ). Tryptone, yeast extract, and agar were from BD Sciences (Franklin Lakes, NJ). Isopropyl 1-thio-β-D-galactopyranoside (IPTG) was from Invitrogen (Carlsbad, CA). [γ-32P]ATP (3 mCi/nmol) and γ-32Pi were from PerkinElmer (Waltham, MA). All other chemicals including α-KG, Fe(NH4)2(SO4)2, were of reagent grade. Purified Kdo2-lipid A was obtained from Avanti Polar Lipids (Alabaster, AL).
Chung H.S., Yang E.G., Hwang D., Lee J.E., Guan Z, & Raetz C.R. (2014). Kdo hydroxylase is an inner core assembly enzyme in the Ko-containing lipopolysaccharide biosynthesis. Biochemical and biophysical research communications, 452(3), 789-794.
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3

Chromatographic Analysis of Biomolecules

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Chloroform, methanol, and silica gel 60 (0.25 mm) thin layer chromatography plates, as well as high-performance analytical thin layer chromatography plates were purchased from EMD Chemicals Inc. (Gibbstown, NJ). Tryptone, yeast extract, and agar were purchased from Becton, Dickinson and Co. (Franklin Lakes, NJ). Isopropyl 1-thio-β-D-galactopyranoside (IPTG) was purchased from Invitrogen Corp. (Carlsbad, CA). [γ−32P]ATP (3 mCi/nmol) and Phosphophorous-32 were from PerkinElmer Life and Analytical Sciences Inc. (Waltham, MA). All other chemicals, including α-KG, FeCl3 and Fe(NH4)2(SO4)2, were reagent grade and were purchased from either Sigma-Aldrich or Mallinckrodt Baker Inc. (Phillipsburg, NJ). Purified Kdo2-lipid A was obtained from Avanti Polar Lipids Inc. (Alabaster, AL).
Joo S.H., Pemble CW I.V., Yang E.G., Raetz C.R, & Chung H.S. (2018). Biochemical and structural insights into an Fe(II)/α-ketoglutarate/O2 dependent dioxygenase, Kdo 3-hydroxylase (KdoO). Journal of molecular biology, 430(21), 4036-4048.
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