Dtsp20

Manufactured by R&D Systems

Sourced in United States

The DTSP20 is a laboratory instrument designed for the detection and quantification of proteins. It utilizes a spectrophotometric method to measure the absorbance of samples, allowing for the determination of protein concentration.

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Lab products found in correlation

Eia 1474r, by DRG International (1 mentions) Ca125, by R&D Systems (1 mentions)

3 protocols using dtsp20

Genotyping of SNPs Associated with Tumor Markers

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Sixteen SNPs (in FUT3, FUT2, FUT6, ABO, GAL3ST2, MUC16, B3GNT3, FAM3B and THBS2) associated with tumor marker levels (CA19-9, CEA, CA125, thrombospondin-2) from prior studies^{16 (link)-18 (link), 20 (link)} were genotyped (Table S1).
All serum samples were assayed in duplicate and analyzed randomly. New serum aliquots were used for all measurements. Tumor marker levels were measured by ELISA; CA19-9 (EIA-1474R, DRG International, NJ, USA), CEA (EIA-1868R, DRG International), CA125 (CA125, Quantikine; R&D Systems, Minneapolis, MN, USA), thrombospondin-2 (DTSP20, Quantikine; R&D Systems). Since CA125 levels are higher prior to menopause³⁰, CA125 levels in women were measured only in those age >55 (n=174). An internal reference serum was measured in duplicate for each ELISA plate. Coefficients of variation calculated using a serum reference sample were 9.0%, 6.9% and 7.9%, for CA19-9, CA125 and thrombospondin-2, respectively. Further description of methods are provided in Supplemental Materials.

Abe T., Koi C., Kohi S., Song K.B., Tamura K., Macgregor-Das A., Kitaoka N., Chuidian M., Ford M., Dbouk M., Borges M., He J., Burkhart R., Wolfgang C.L., Klein A.P., Eshleman J.R., Hruban R.H., Canto M.I, & Goggins M. (2019). Gene Variants That Affect Levels of Circulating Tumor Markers Increase Identification of Patients with Pancreatic Cancer. Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association, 18(5), 1161-1169.e5.

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Plasma TSP2 Quantification ELISA

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Plasma samples were collected at study enrolment and subjects were nonfasting. Peripheral venous blood was collected using EDTA tubes, centrifuged immediately and plasma aliquots were stored at −80°C until required without additional freeze–thaw cycles. Absolute concentration of plasma TSP2 levels were measured using a quantitative enzyme linked immunosorbent assay (DTSP20; R&D Systems, MN, United States). Based on duplicative measurements, mean coefficient of variation was 2% for intra-assay and 7.2% for inter-assay variability.

Dittrich A.M., Mienert J., Pott J., Engels L., Sinning C., Hennigs J.K., Klose H, & Harbaum L. (2023). Clinical phenotyping of plasma thrombospondin-2 reveals relationship to right ventricular structure and function in pulmonary hypertension. ERJ Open Research, 9(2), 00528-2022.

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Quantitative ELISA Measurement of Thrombospondin-2 and CA 19-9 in Serum

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The serum levels of human thrombospondin-2 were determined by quantitative sandwich enzyme-linked immunosorbent assay (ELISA) using a commercially available kit (DTSP20, R&D Systems, Minneapolis, MN, USA). The assay was performed according to the manufacturer’s instructions. All measurements were performed in technical duplicates. First or second thaw cycles were used. Samples were diluted four- to tenfold in calibrator diluent. Optical density was determined using a Multiskan GO microplate spectrophotometer (Thermo Fisher Scientific Oy, Vantaa, Finland) at 450 nm and 540 nm to correct for optical imperfections. Sample concentrations were determined from a standard curve of the positive controls included in the kit. A four-parameter logistic regression standard curve was generated using MyAssays online data analysis tool (MyAssays Ltd., accessed June 2020, http://www.myassays.com/four-parameter-logistic-curve.assay). Four samples had a higher concentration than the range of the standard curve at a tenfold dilution, and values were extrapolated by extending the standard curve.
Serum levels of CA 19-9 were measured in a clinical laboratory (Department of Clinical Chemistry and Pharmacology, University and Regional Laboratories Region Skåne, Sweden) using a clinically accredited immunoassay (Ref.11776193 122 Cobas/Roche).

Byrling J., Hilmersson K.S., Ansari D., Andersson R, & Andersson B. (2021). Thrombospondin-2 as a diagnostic biomarker for distal cholangiocarcinoma and pancreatic ductal adenocarcinoma. Clinical & Translational Oncology, 24(2), 297-304.

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