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Rabbit anti synj 1

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Rabbit anti-Synj-1 is a primary antibody that specifically binds to the Synj-1 protein, which is involved in synaptic vesicle recycling. This antibody can be used in various research applications, such as Western blotting, immunohistochemistry, and immunoprecipitation, to detect and study the expression and localization of Synj-1 in biological samples.

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Lab products found in correlation

Cy3 conjugated anti hrp, by Jackson ImmunoResearch (2 mentions) Mouse anti dynamin, by BD (2 mentions) Mouse anti gluriia, by Developmental Studies Hybridoma Bank (2 mentions) Mouse anti bruchpilot nc82, by Developmental Studies Hybridoma Bank (2 mentions) Mouse anti dlg 4f3, by Developmental Studies Hybridoma Bank (2 mentions)

2 protocols using rabbit anti synj 1

1

Immunolabeling of Drosophila Larval Synaptic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Third-instar larvae were dissected and fixed in 4% paraformaldehyde (except Bouin’s fixative was used for GluRIIA staining). Fixed samples were washed with 0.1% triton X-100 in PBS (PBST) then blocked with 5% normal goat serum (NGS) in PBST. Primary antibodies used to label dissected larvae were diluted in blocking solution and used as following: rabbit anti-p-Synj, 1:200030 (link); guinea-pig anti-Endo(GP69), 1:20040 (link), guinea-pig anti-Dap160, 1:100033 (link); rabbit anti-synaptotagmin, 1:100069 (link), rabbit anti-Mnb, 1:400; rabbit anti-Synj-1, 1:200; mouse anti-dynamin, 1:200 (BD Transduction Laboratories); Cy3 conjugated anti-HRP, 1:100 (Jackson ImmunoResearch); mouse anti-bruchpilot (NC82), 1:10; mouse anti-GluRIIA, 1:50 and mouse anti-Dlg (4F3), 1:500 (Developmental Studies Hybridoma Bank). Secondary antibodies used were Alexa-488, 555 or 405 conjugated, 1:250 (Invitrogen).
Chen C.K., Bregere C., Paluch J., Lu J., Dickman D.K, & Chang K.T. (2014). Activity-dependent facilitation of Synaptojanin and synaptic vesicle recycling by the Minibrain kinase. Nature communications, 5, 4246.
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2

Immunolabeling of Drosophila Larval Synaptic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Third-instar larvae were dissected and fixed in 4% paraformaldehyde (except Bouin’s fixative was used for GluRIIA staining). Fixed samples were washed with 0.1% triton X-100 in PBS (PBST) then blocked with 5% normal goat serum (NGS) in PBST. Primary antibodies used to label dissected larvae were diluted in blocking solution and used as following: rabbit anti-p-Synj, 1:200030 (link); guinea-pig anti-Endo(GP69), 1:20040 (link), guinea-pig anti-Dap160, 1:100033 (link); rabbit anti-synaptotagmin, 1:100069 (link), rabbit anti-Mnb, 1:400; rabbit anti-Synj-1, 1:200; mouse anti-dynamin, 1:200 (BD Transduction Laboratories); Cy3 conjugated anti-HRP, 1:100 (Jackson ImmunoResearch); mouse anti-bruchpilot (NC82), 1:10; mouse anti-GluRIIA, 1:50 and mouse anti-Dlg (4F3), 1:500 (Developmental Studies Hybridoma Bank). Secondary antibodies used were Alexa-488, 555 or 405 conjugated, 1:250 (Invitrogen).
Chen C.K., Bregere C., Paluch J., Lu J., Dickman D.K, & Chang K.T. (2014). Activity-dependent facilitation of Synaptojanin and synaptic vesicle recycling by the Minibrain kinase. Nature communications, 5, 4246.
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