To pinpoint the morphological changes within the sample as a function of the extent of GSH denaturation, scanning electron microscopy (SEM) were taken using the HITACHI FE-SEM SU8010 instrument at 10.0 kV accelerating voltage and 5 milliamps. As the detection system is based on visual changes in the solution hues, the LAMBDA 950 UV/Vis/NIR Spectrophotometer by PerkinElmer was employed in absorbance mode to quantify the max peak wavelength shifts. The parameters used for UV-Vis spectra consisted of a 2 nm slit width and scan speed of 480.23 nm/min over a 400 nm range starting at 800 nm. To formally quantify morphological changes in the system due to aggregation of the gold nanoparticles, a dynamic light scattering technique was used to elucidate the size and size distribution of the gold nanoparticle, GSH and APTES systems via the Nanotrac Wave by Microtrac. The 60 second trials runs on the Nanotrac Wave by Microtac used a 780 nm laser diode. FTIR spectroscopy was employed to determine denaturation of GSH via a transmission spectrum over the range of 3000–1000 (cm−1), using a 0.5 (cm^−1) resolution. This was achieved using the PerkinElmer Spectrum Two Spectrophotometer and Spectrum software application version 10.03.06.0100.
Spectrum two spectrophotometer
Manufactured by PerkinElmer
Sourced in United States, United Kingdom
The Spectrum Two spectrophotometer is a high-performance analytical instrument designed for laboratory use. It measures the absorption or transmission of light by a sample over a specific range of wavelengths, providing quantitative data about the chemical composition of the sample. The Spectrum Two offers accurate and reliable performance for a variety of spectroscopic applications.
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Lab products found in correlation
Dsc 6000 calorimeter, by PerkinElmer (4 mentions)
Uv 1700 uv vis spectrophotometer, by Shimadzu (3 mentions)
240c elemental analyzer, by PerkinElmer (2 mentions)
D8 instrument, by Bruker (2 mentions)
Rf 5301pc spectrofluorometer, by Shimadzu (2 mentions)
Mcp photomultiplier, by Hamamatsu Photonics (2 mentions)
Utar ftir spectrophotometer, by PerkinElmer (2 mentions)
Nanotrac wave, by Microtrac (1 mentions)
Su8010 fe sem, by Hitachi (1 mentions)
Lambda 950 uv vis nir spectrophotometer, by PerkinElmer (1 mentions)
Tga sdta 851e, by Mettler Toledo (1 mentions)
240c elemental, by PerkinElmer (1 mentions)
Jnm ecz400s l1, by JEOL (1 mentions)
V 630 spectrophotometer, by Jasco (1 mentions)
300 mhz nmr spectrometer, by Bruker (1 mentions)
Mpms xl 5 squid susceptometer, by Quantum Design (1 mentions)
240c elemental analyser, by PerkinElmer (1 mentions)
Uv 1700 spectrophotometer, by Shimadzu (1 mentions)
Tecnai g2 spirit twin, by Thermo Fisher Scientific (1 mentions)
Lb 550, by Horiba (1 mentions)
28 protocols using spectrum two spectrophotometer
1
Characterization of Gold Nanoparticle Aggregation
2
FT-IR Characterization of CS/PEG CPC
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Fourier transform infrared (FT-IR) spectra of CS/PEG CPC were measured in the range from 1600 to 400 cm−1. FT-IR spectra were recorded on a PerkinElmer Spectrum Two spectrophotometer (Waltham, MA, USA) for solid and liquid samples.
3
Characterization of Materials by FT-IR
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FT-IR spectra were recorded on a PerkinElmer spectrum two spectrophotometer equipped with an ATR accessory.
4
Thermal and Spectroscopic Analysis of Compounds
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Thermogravimetric
(TG) analysis was carried out with a Mettler-Toledo TGA/SDTA851e thermobalance
using aluminum crucibles. All experiments were performed in a dynamic
oxygen atmosphere with a flow rate of 200 cm3 min–1. Heating rates of 5 K min–1 were used for all
investigations. ATR (attenuated total reflectance) Fourier Transform
Infrared spectra (FT-IR) were collected with a Perkin-Elmer Spectrum
Two spectrophotometer in the spectral range 4500–450 cm–1. Results of the analysis were used for identification
purposes and to assess thermal stability and are listed in Supporting
Information,Figures S1–S4 .
(TG) analysis was carried out with a Mettler-Toledo TGA/SDTA851e thermobalance
using aluminum crucibles. All experiments were performed in a dynamic
oxygen atmosphere with a flow rate of 200 cm3 min–1. Heating rates of 5 K min–1 were used for all
investigations. ATR (attenuated total reflectance) Fourier Transform
Infrared spectra (FT-IR) were collected with a Perkin-Elmer Spectrum
Two spectrophotometer in the spectral range 4500–450 cm–1. Results of the analysis were used for identification
purposes and to assess thermal stability and are listed in Supporting
Information,
5
Spectroscopic and Thermal Analysis of Residues
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The dried solid residues obtained after the solubility determination procedure were subjected to Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) measurements. For this purpose, the Perkin Elmer Spectrum Two spectrophotometer (Waltham, MA, USA) equipped with an attenuated total reflection (ATR) device and the DSC 6000 calorimeter from PerkinElmer (Waltham, MA, USA) were used. The calorimetric measurements were conducted with a heating rate of 5 K/min and a 20 mL/min nitrogen flow to create an inert atmosphere. The samples were placed in standard aluminum pans and the DSC apparatus was calibrated using indium and zinc standards prior to the measurements.
6
Biochar Characterization: Comprehensive Analysis
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The elemental compositions of biochars were determined by dynamic flash combustion using a Vario MICRO elemental analyzer. Oxygen content was determined by calculating the difference between the measured mass and the mass accounted for carbon, hydrogen, and nitrogen. The pH of the biochar was measured in the supernatant of the aqueous solution of biochar (solid-water ratio was 1:20). Pore structure of biochars was characterized by nitrogen adsorption at 77K with automated surface area and pore size analyzer. The specific surface area was determined from the adsorption isotherms using the BET equation. The FT-IR spectra of the biochars were recorded on a Perkin Elmer Spectrum Two spectrophotometer. The cation exchange capacity (CEC) of the biochars was measured using sodium acetate for exchange, and determining the Na+ content using a flame spectrophotometer. The point of zero charge of biochar pH (pHZPC) was determined based on a protocol reported previously [16 ]. The morphology of the biochars was observed on an S4800 scanning electron microscope (SEM).
7
Elemental and Spectroscopic Analysis of Compounds
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Elemental analyses (carbon, hydrogen and nitrogen) were performed using a PerkinElmer 240C elemental analyzer. IR spectra in KBr (4500 to 500 cm−1) were recorded with a PerkinElmer Spectrum Two spectrophotometer. Electronic spectra in DMF were recorded on a Shimadzu UV-1700 UV-vis spectrophotometer. The 1H NMR spectra at 400 MHz were recorded in DMSO-d6 on a JEOL-JNM-ECZ400 S/L1.
8
Halloysite-Reinforced Epoxy Resin Composites
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Araldite F, solvent-free and unmodified bisphenol- A epoxy resin was purchased from Huntsman group. Its epoxy contents are 5.20–5.35 Equiv/Kg (ISO 3001), having a density range from 1.15–1.20 g/cm3. Acrylic acid (AA, >99% purity) was supplied by power chemical industries (PVT) LTD. Pakistan. Benzimidazole (>99% purity) was purchased from Alfa Aesar (A Johnson Matthey Company) Karlsruhe Germany. Halloysite nanotubes (HNTs) were purchased from natural nano, United States of America. Potassium persulfate (KPS) and 2-hydroxy-2-methylpropiohenone (analytical grade) were used as a thermal curing agent and a photo-initiator. Deionized water (DDW) was used in all experiments. Potassium hydroxide (KOH, >99% purity) was used as a standard for titration (0.1 N). FTIR spectra were recorded on Perkin-Elmer Spectrum two spectrophotometer.
9
Multitechnique Characterization of Materials
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Elemental analysis (carbon, hydrogen and nitrogen) was performed using a Perkin-Elmer 240C elemental analyzer. IR spectrum in KBr (4500–500 cm−1) was recorded with a Perkin-Elmer Spectrum Two spectrophotometer. Electronic spectra in DMF were recorded on a JASCO V-630 spectrophotometer. The powder XRD data were collected on a Bruker D8 Advance X-ray diffractometer using Cu Kα radiation (λ = 1.548 Å) generated at 40 kV and 40 mA. The PXRD spectrum was recorded in a 2θ range of 5–50° using 1-D Lynxeye detector at ambient conditions.
10
Comprehensive Characterization of Novel Compound
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Elemental analyses (carbon, hydrogen and nitrogen) were performed using a PerkinElmer 240C elemental analyzer. IR spectrum in KBr (4500–500 cm−1) was recorded with a PerkinElmer Spectrum Two spectrophotometer. Electronic spectrum in CH3CN was recorded on a Shimadzu UV-1700 UV-vis spectrophotometer. The magnetic susceptibility measurement was performed with an EG and PAR vibrating sample magnetometer, model 155 at room temperature (300 K) in a 5000 G magnetic field, and diamagnetic corrections were performed using Pascal's constants. 1H NMR spectrum in DMSO-d6 solvent was recorded in a JEOL 400 MHz NMR instrument (Fig. S4, ESI† ).13C NMR spectrum of the complex in DMSO-d6 solvent was recorded in a BRUKER 300 MHz NMR Spectrometer (Fig. S5, ESI† ).
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