Rapamycin was obtained from LC Labs. (-)-OC, extracted from EVOO, was obtained from Dr. Alexios-Leandros Skaltsounis at the University of Athens Department of Pharmacology. The structure and purity (97%) of (-)-oleocanthal was determined by HPLC and 1H-NMR analysis. Acridine orange was obtained from Life Technologies. The Promokine Apoptotic/Necrotic/Healthy cell detection kit and Cerulenin were obtained from Fisher Scientific. The Sphingomyelinase Fluorometric Assay kit was obtained from Cayman Chemicals. Primary antibodies specific for cleaved caspase-3, cleaved PARP, phospho-p44/42 (Thr202/Tyr204), total p44/42, β-actin, and phospho-Rb (Ser 608) were obtained from Cell Signaling Technology. Antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and Hsp70 were obtained from Santa Cruz Biotechnology.
Promokine apoptotic necrotic healthy cell detection kit
Manufactured by Thermo Fisher Scientific
The Promokine Apoptotic/Necrotic/Healthy Cell Detection Kit is a fluorescence-based assay designed to detect and quantify apoptotic, necrotic, and healthy cells in a sample. The kit uses Annexin V and Propidium Iodide as markers to identify the different cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Cerulenin, by Thermo Fisher Scientific (2 mentions)
Sphingomyelinase fluorometric assay kit, by Cayman Chemical (2 mentions)
Phospho rb ser 608, by Cell Signaling Technology (2 mentions)
Antibodies against glyceraldehyde 3 phosphate dehydrogenase gapdh and hsp70, by Santa Cruz Biotechnology (2 mentions)
Total p44 42, by Cell Signaling Technology (2 mentions)
Acridine orange, by Thermo Fisher Scientific (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
2 protocols using promokine apoptotic necrotic healthy cell detection kit
1
Rapamycin and Oleocanthal Synergistic Effects
2
Rapamycin and Oleocanthal Synergistic Effects
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