Tumor tissue analogs were collected and fixed in 10% formalin for 2 h, washed with PBS and dehydrated twice with 50 and 70% ethanol for 15 min each. The dehydrated TTA were transferred to the cryomold (Thermo Fisher Scientific Inc., Waltham, MA) and embedded in HistoGel™ (Thermo Scientific™, Kalamazoo, MI) liquefied by heating at 60° ± 5°C. The TTA containing Cryomolds® were subsequently solidified ice for 10 min. The HistoGel blocks with TTA were wrapped within a piece of Bio-Wrap™ (Leica Biosystems, Buffalo Grove, IL) and placed into tissue biopsy cassette (Thermo Fisher Scientific) for processing. Tissue biopsy cassettes containing HistoGel blocks were dehydrated by grades of alcohol and then cleaned with xylene and impregnated with paraffin. Processed HistoGel blocks were then removed from the Bio-Wrap and placed in wax (Paraplast X-TRA®, Sigma-Aldrich) to prepare paraffin blocks. Sections (4–5 μm) were cut with a microtome (Leica Biosystems).
Histogel
Manufactured by Leica
Sourced in Germany
HistoGel is a specialized laboratory product designed for the preparation and processing of tissue samples. It serves as a solidifying medium that aids in the handling and sectioning of delicate or fragile tissue specimens during histological analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using histogel
1
Formalin-Fixed Tissue Analog Embedding
2
Histological Comparison of Organoids and Biopsies
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Formalin-fixed paraffin-embedded blocks and hematoxylin and eosin-stained slides of organoids and biopsy tissues were made for the eight organoid-tissue pairs, and the histomorphological features of the organoids and biopsies were compared. Organoids grown in Matrigel for histology were fixed in 10% paraformaldehyde for 1 hour. The organoids were pre-embedded in Histogel (Richard-Allan Scientific HG-4000-012; Thermo Fisher, Waltham, MA, USA), and returned to fixative in 4% paraformaldehyde overnight. After fixing, the organoids were washed by phosphate buffered saline, and hematoxylin inked. Organoids embedded in Histogel were processed by automated tissue processor (Peloris II; Leica Biosystems, Wetzlar, Germany), and embedded into a paraffin block (Histocentre 3, Shandon). Samples were sectioned at 4 μm (Microtome RM2255; Leica Biosystems) onto poly-l-lysine coated slides, and air-dried at 45°C overnight for any subsequent routine hematoxylin-eosin staining. The histology of all tissue and organoid specimens was reviewed independently by a single experienced pancreaticobiliary pathologist (H.K.).
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