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Neb standard buffer 4

Manufactured by New England Biolabs
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NEB Standard Buffer 4 is a general-purpose buffer solution formulated for use in various molecular biology applications. Its core function is to provide a stable and consistent environment for enzymatic reactions.

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Lab products found in correlation

Hek293t cell line, by American Type Culture Collection (2 mentions) Anti flag antibody, by Merck Group (2 mentions) Dh5α competent cells, by Thermo Fisher Scientific (2 mentions) Quantitect sybr green pcr master mix, by Qiagen (2 mentions) Anti phospho iκbα, by Cell Signaling Technology (2 mentions) Ecorv, by New England Biolabs (2 mentions) Anti ha, by Cell Signaling Technology (2 mentions) Anti β actin antibody, by Abcam (2 mentions) Anti a20 antibody, by Thermo Fisher Scientific (2 mentions) Pcs2tal3dd, by Addgene (2 mentions) Pcs2tal3rr, by Addgene (2 mentions) Nlaiii, by New England Biolabs (2 mentions)

Close spelling variants of this product

NEB buffer (13 citations) Standard buffer (11 citations) NEB Buffer 4 (9 citations) Buffer 4 (9 citations)

2 protocols using neb standard buffer 4

1

A20 Regulation of NF-κB Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-FLAG antibody was purchased from Sigma (St. Louis, MO); anti-HA and anti-phospho-IκBα antibodies were purchased from Cell Signaling (Danvers, MA); anti-β-actin antibody was purchased from Abcam (Cambridge, MA); anti-A20 antibody was purchased from Ebioscience (San Diego, CA). HEK293T cell line was purchased and maintained per instructions from American Type Culture Collection (ATCC; Manassas, VA). Golden Gate cloning kit and expression vectors, pCS2TAL3DD and pCS2TAL3RR, were purchased from Addgene (Cambridge, MA). DH5α competent cells were purchased from Life Technologies (Grand Island, NY). QuantiTect SYBR Green PCR Master Mix was purchased from QIAGEN (Valencia, CA). Restriction enzymes NlaIII, EcoRV, and NEB Standard Buffer 4 were purchased from New England Biolabs (Ipswich, MA).
Wang S., Wen F., Tessneer K.L, & Gaffney P.M. (2016). TALEN-mediated enhancer knockout influences TNFAIP3 gene expression and mimics a molecular phenotype associated with systemic lupus erythematosus. Genes and immunity, 17(3), 165-170.
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2

A20 Regulation of NF-κB Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-FLAG antibody was purchased from Sigma (St. Louis, MO); anti-HA and anti-phospho-IκBα antibodies were purchased from Cell Signaling (Danvers, MA); anti-β-actin antibody was purchased from Abcam (Cambridge, MA); anti-A20 antibody was purchased from Ebioscience (San Diego, CA). HEK293T cell line was purchased and maintained per instructions from American Type Culture Collection (ATCC; Manassas, VA). Golden Gate cloning kit and expression vectors, pCS2TAL3DD and pCS2TAL3RR, were purchased from Addgene (Cambridge, MA). DH5α competent cells were purchased from Life Technologies (Grand Island, NY). QuantiTect SYBR Green PCR Master Mix was purchased from QIAGEN (Valencia, CA). Restriction enzymes NlaIII, EcoRV, and NEB Standard Buffer 4 were purchased from New England Biolabs (Ipswich, MA).
Wang S., Wen F., Tessneer K.L, & Gaffney P.M. (2016). TALEN-mediated enhancer knockout influences TNFAIP3 gene expression and mimics a molecular phenotype associated with systemic lupus erythematosus. Genes and immunity, 17(3), 165-170.
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