Massarray typer software v4

Based on GWAS results of the European and Japanese PBC cohorts, we selected the most relevant 22 tag SNPs, at the chromosomal region (chr3:159619322–159800889) surrounding IL12A, from the 1000 Genomes Project for Asian populations with minor allele frequency >2% by Haploview v4.2.^{31 (link)} The selected 22 SNPs around the genomic region of IL12A were genotyped in a cohort of Chinese Han individuals (586 cases/726 controls) by MassArray iPLEX.
The genomic DNA of each sample was extracted using a QIAamp DNA mini kit (Qiagen, Hilden, Germany), and SNP genotyping was performed by the MassArray iPLEX system (Sequenom, San Diego, CA, USA) at Beijing DNALead Co. LTD. All procedures were performed according to the manufacturer's instructions. Approximately 10 ng of genomic DNA was amplified by multiplex polymerase chain reaction (PCR) and the amplicons were subjected to locus-specific single-base extension reactions. The extended products were desalted and transferred to a 384-element SpectroCHIP array. Allele detection was performed using MALDI-TOF mass spectrometry, and the mass spectrograms were analyzed by the MassArray TYPER software v4.0 (Sequenom).
The procedures for SNP genotyping and Hardy–Weinberg equilibrium analysis were performed as previously reported.^{32 (link)}

The genomic DNA of each sample was extracted using QIAamp DNA mini kit (Qiagen, German), and SNP genotyping was performed by the MassArray iPLEX system (Sequenom, USA) at Beijing DnaLead Co. LTD. All procedures were performed according to the manufacturer’s instructions. Approximately 10 ng of genomic DNA was amplified by multiplex PCR and the amplicons subjected to locus-specific single-base extension reactions. The extended products were desalted and transferred to a 384-element SpectroCHIP array. Allele detection was performed using MALDI-TOF mass spectrometry, and the mass spectrograms were analyzed by the MassArray TYPER software v4.0 (Sequenom, USA). 6 SNPs in the coding sequence of HELZ2 were selected for genotyping in the 2 cohorts of Chinese Han (385 cases/726 controls and 201 cases/726 controls) by MassArray iPLEX.