A549 (human non-small cell lung carcinoma) cell line was purchased from ATCC®, CORL-105 (Caucasian lung adenocarcinoma) cell line was purchased from ECACC® (The European Collection of Cell Cultures), supplied by Sigma-Aldrich (St. Louis, MO, USA), and lentivirus producer cell line (HEK-293T) was purchased from DSMZ. Cells were cultured in DMEM (Sigma Aldrich) (A549 and HEK293T) or RPMI 1640 (Lonza, Basel, Switzerland) (CORL-105) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich), 1% penicillin/streptomycin, 4 mM L-glutamine in a humidified atmosphere with 5% CO2 at 37 °C. Cell lines were cultured according to ATCC/ECACC/DSMZ protocols. Cells were maintained in a humidified atmosphere with 5% CO2 and 95% air (normoxia) at 37 °C. For hypoxic conditions, cells were incubated in a hypoxic incubator (BINDER, CB53) with a humidified atmosphere of 5% CO2 and 1% O2 balanced with N2 (hypoxia). Exponentially growing cells were used throughout the study. All cell cultures were regularly checked for mycoplasma contamination by PCR and proved negative.
Cor l105
Manufactured by Merck Group
Sourced in Switzerland, United States, United Kingdom
COR-L105 is a laboratory equipment product from Merck Group. It is designed for general laboratory use. The core function of COR-L105 is to provide a reliable and consistent environment for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Nci h1299, by American Type Culture Collection (3 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Hek293t, by Leibniz Institute DSMZ (1 mentions)
Rpmi 1640, by Lonza (1 mentions)
Hek293t, by Lonza (1 mentions)
Nci h23, by American Type Culture Collection (1 mentions)
Hcc827, by American Type Culture Collection (1 mentions)
Nci h460, by American Type Culture Collection (1 mentions)
Calu 3, by American Type Culture Collection (1 mentions)
Nci h358, by American Type Culture Collection (1 mentions)
Nci h2170, by American Type Culture Collection (1 mentions)
Nci h1975, by American Type Culture Collection (1 mentions)
Nci h1437, by American Type Culture Collection (1 mentions)
Nci h441, by American Type Culture Collection (1 mentions)
Nci h2347, by American Type Culture Collection (1 mentions)
Nci h1734, by American Type Culture Collection (1 mentions)
Nci h1568, by American Type Culture Collection (1 mentions)
Nci h2087, by American Type Culture Collection (1 mentions)
Nci h2106, by American Type Culture Collection (1 mentions)
Human serum, by Merck Group (1 mentions)
4 protocols using cor l105
1
Culturing Lung Cancer Cell Lines
2
NSCLC Cell Line Panel for Research
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The human NSCLC cell lines HCC827, NCI‐H1437, NCI‐H1734, NCI‐H358, NCI‐H1781, NCI‐H2170, NIC‐H1650, NCI‐H2106, NCI‐H2087, NCI‐H2347, NCI‐H441, Hs 618.T, NCI‐H1299, NCI‐H460, NCI‐H1975, NCI‐H1568, NCI‐H23, Calu‐3, and A549 were obtained from the American Type Culture Collection (Manassas, VA, USA). The human NSCLC cell line COR‐L105 was purchased from Sigma‐Aldrich, and human NSCLC cell line HCC‐15 was purchased from Creative Dynamics (Shirley, NY , USA). The HS618.T cell line was cultured in Dulbecco's Modified Eagle's medium, supplemented with 10% FBS. A549 was cultured in F‐12K medium, supplemented with 10% FBS. NCI‐H2106 was cultured in HITES medium supplemented with 5% FBS. Calu‐3 was cultured in Eagle's Minimum Essential Medium supplemented with 10% FBS. NCI‐H2087 was cultured in RPMI‐1640 medium supplemented with 5% FBS. All other cell lines were maintained in RPMI‐1640 medium supplemented with 10% FBS. Cells were grown at 37 °C in a humidified 5% CO2 : 95% air atmosphere.
3
Cell Lines for NSCLC and Glioblastoma Research
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The human cerebral microvascular endothelial cell line (hCMEC/D3) was donated by Professor Couraud (Institute of Cochin, INSERM, Paris, France) [14 (link)] and cultured in endothelial basal medium-2 (EGM-2) (Lonza, Germany) supplemented with 2% human serum (Sigma, UK). Primary NSCLC cells (COR-L105) were purchased from Sigma, UK; metastatic NSCLC cells from cervical lymph node (NCI-H1299) from ATCC, UK and low-passage biopsy-derived brain-metastatic NSCLC cells, obtained from a patient with lung-brain metastatic cancer (SEBTA-001) as well as a biopsy-derived primary glioblastoma (GBM) cell line (UP-007) both established “in house”. Cell lines were maintained at 5% CO2 and in a humidified atmosphere at 37 °C. All lines were subjected to routine mycoplasma testing, and cell authentication [15 (link)].
4
Cell Culture Protocol for Lung Cancer Research
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The human cerebral microvascular endothelial cell line (hCMEC/D3) was donated by Professor Pierre Olivier Couraud (Institute of Cochin, INSERM, Paris, France).36 hCMEC/D3 cells were cultured in endothelial basal medium-2 (EBM-2) supplemented vascular endothelial growth factor (VEGF), human epidermal growth factor (hEGF), R3-insulin-like growth factor-1 (R3-IGF-1), ascorbic acid, hydrocortisone, human fibroblast growth factor-beta (hFGF-β), heparin (all from Lonza), and 2% human serum (Biosera). Primary NSCLC cells (A549 and COR-L105) were purchased from Sigma and metastatic NSCLC cells from cervical lymph nodes (NCI-H1299) from ATCC. Low-passage brain-metastatic NSCLC cell lines (SEBTA-001 and SEBTA-005) were established in house using biopsies from patients with lung-brain secondary tumors. All NSCLCs were cultured in Dulbecco′s modified Eagle medium supplemented with 2% human serum (Biosera) and maintained in 5% CO2 and humidified atmosphere at 37°C. All cell lines were subjected to routine mycoplasma testing, utilizing a kit from Lonza. Cell authentication was conducted using a microfluidic electrophoresis system incorporating an Agilent 2100 Bioanalyzer (Agilent Technologies\) to analyze STR-PCR fragments from 10 human genomic loci of human cell lines.37 (link)
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