Sorvell st40r centrifuge

Example 14

A cell broth (350 g) containing microbial cells (Schizochytrium sp.) was pasteurized at 60° C. for 1 hour. A demulsified lysed cell composition was formed by adding 8%, by weight broth, of phosphoric acid and heating the broth to 90° C. for 3 hours. The microbial oil was separated from the demulsified lysed cell composition by adding a 50 wt % NaOH solution to adjust the pH to 7-8.5 and, after a few hours at 90° C., centrifuging (Thermo Scientific Sorvell ST40R Centrifuge) the composition at 8000 RPM for 5 minutes to recover the crude oil. A 45 g sample of this composition yielded 0.5 mL of crude oil.

Example 10

A cell broth (350 g) containing microbial cells (Schizochytrium sp.) was pasteurized at 60° C. for 1 hour. A demulsified lysed cell composition was formed by adding 4%, by weight broth, of a 70 wt % nitric acid solution and heating the broth to 90° C. for 1 hour. The microbial oil was separated from the demulsified lysed cell composition by adding a 50 wt % NaOH solution to adjust the pH to 7-8.5 and centrifuging (Thermo Scientific Sorvell ST40R Centrifuge) the composition at 8000 RPM for 5 minutes to provide a crude oil, which yielded 82% DHA (by DHA weight).

Example 2

A cell broth (300 g) containing microbial cells (Schizochytrium sp.) was pasteurized at 60° C. for 1 hour. A demulsified lysed cell composition was formed by adding a 20% H₂SO₄ by weight solution to adjust the pH of the cell broth to 2 and then heating the broth to 90° C. and holding for 45 hours. The microbial oil was separated from the demulsified lysed cell composition by adding a 50 wt % NaOH solution to adjust the pH of the composition to 7.5-8.5 and then centrifuging (Thermo Scientific Sorvell ST40R Centrifuge) the composition at 8000 RPM for 5 minutes to provide a crude oil, which yielded 51.62% DHA (by DHA weight).

Example 10

A cell broth (300 g) containing microbial cells (Schizochytrium sp.) was pasteurized at 60° C. for 1 hour. The cell broth was agitated at a speed of 180 RPM and heated to 60° C. The cells were lysed by adding a 50 wt % NaOH solution to pH adjust the unwashed broth to 7-7.5 and Alcalase® 2.4 FG (available from, Novozymes (Franklinton, N.C.)) in an amount of 0.5% based on broth weight. While maintaining the agitation, the pH of the lysed cell composition was adjusted to 10-11 by adding a 12.5 wt % NaOH solution. Simultaneously, SDS powder was added in an amount of 1% by weight of the lysed cell composition and the composition heated to 90° C. After 2.5 hours at 90° C., the composition was centrifuged (Thermo Scientific Sorvell ST40R Centrifuge) at 8000 RPM for 5 minutes to provide a crude oil, which yielded 92% DHA (by DHA weight).

Example 21

A cell broth (300 g) containing microbial cells (Schizochytrium sp.) was pasteurized at 60° C. for 1 hour. A demulsified lysed cell composition was formed by: adding a 12.5 wt % NaOH solution to the cell broth to adjust the pH to 11, adding 2% NaCl, by weight broth, and heating the composition to 90° C. After 25 hours at 90° C., the microbial oil was separated from the demulsified lysed cell composition by centrifuging (Thermo Scientific Sorvell ST40R Centrifuge) the composition at 8000 RPM for 5 minutes to provide crude oil, which yielded 71% DHA (by DHA weight).

Example 9

A cell broth (300 g) containing microbial cells (Schizochytrium sp.) was pasteurized at 60° C. for 1 hour. The cell broth was agitated at a speed of 180 RPM and heated to 60° C. The cells were lysed by adding a 50 wt % NaOH solution to pH adjust the unwashed broth to 7-7.5 and Alcalase® 2.4 FG (available from, Novozymes (Franklinton, N.C.)) in an amount of 0.5% based on broth weight. While maintaining the agitation, the pH of the lysed cell composition was adjusted to 10-11 by adding a 12.5 wt % NaOH solution. Simultaneously, SDS powder was added in an amount of 0.5% by weight of the lysed cell composition and the composition heated to 80-90° C. for 2 hours. After the hold time, solid NaCl in an amount of 2% by weight of the lysed cell composition was added and the composition heated to 90° C. and held for 18 hours. The lysed cell composition was centrifuged (Thermo Scientific Sorvell ST40R Centrifuge) at 8000 RPM for 5 minutes to yield 80% crude oil, which yielded 85% DHA (by DHA weight).

Example 5

A cell broth (300 g) containing microbial cells (Schizochytrium sp.) was pasteurized at 60° C. for 1 hour. The unwashed cell broth was agitated at a speed of 180 RPM and heated to 60° C. The cells were lysed by adding: 50 wt % NaOH to pH adjust the broth to 7-7.5, and Alcalase 2.4 FG (available from Novozymes (Franklinton, N.C.) in an amount of 0.5% based on broth weight. While maintaining the agitation, the pH of the lysed cell composition was adjusted to 4 by adding a 98% H₂SO₄ by weight solution, and then heating the composition to 90° C. After 45 hours at 90° C., the composition was centrifuged (Thermo Scientific Sorvell ST40R Centrifuge) at 8000 RPM for 5 minutes to provide a crude oil, which yielded 81% DHA (by DHA weight). The crude oil has an AV of 14.5.