Rezex rfq fast acid h 8 column

Example 2

HPLC Analyses

HPLC analysis was performed by injecting 6 μL of 0.2 μm filtered culture supernatant onto an Agilent 1100 series system equipped with a Phenomenex Rezex RFQ-Fast Acid H+ (8%) column and a cation H+ guard cartridge (Bio-Rad Laboratories) at 85° C. run using a mobile phase of 0.01 N sulfuric acid at a flow rate of 1.0 mL/min and a diode array detector to measure absorbance at 210 nm. Analytes were identified by comparing retention times and spectral profiles with pure standards. HPLC chromatograms show milli-absorbance units (mAU) on the Y axis and retention time (minutes) on the X axis.

Example 2

HPLC Analyses

HPLC analysis was performed by injecting 6 μL of 0.2 μm filtered culture supernatant onto an Agilent 1100 series system equipped with a Phenomenex Rezex RFQ-Fast Acid H+ (8%) column and a cation H+ guard cartridge (Bio-Rad Laboratories) at 85° C. run using a mobile phase of 0.01 N sulfuric acid at a flow rate of 1.0 mL/min and a diode array detector to measure absorbance at 210 nm. Analytes were identified by comparing retention times and spectral profiles with pure standards. HPLC chromatograms show milli-absorbance units (mAU) on the Y axis and retention time (minutes) on the X axis.