UV–Vis spectra were obtained by using a Shimadzu
Prominence HPLC System (Shimadzu, Kyoto, Japan) coupled with a PDA detector. High-resolution electrospray ionization mass spectra were gathered using a
QSTAR® XL Hybrid LC/MS/MS System (Applied Biosystems, Foster City, CA, USA) equipped with turbo ion spray source, delivering the sample at a rate of 40 µl min
−1. NMR data were collected using a Varian
500-MHz NMR spectrometer with 5 mm Varian
Oneprobe (
1H 500 MHz,
13C 125 MHz). Residual signals from solvents were used for referencing. Semipreparative HPLC was performed using a Phenomenex C18 column (Luna 250 × 10 mm, 5 µm, 4.0 ml min
−1). ECD spectra were obtained on an Aviv Biomedical Model 410 CD Spectrometer.
Miller B.W., Torres J.P., Tun J.O., Flores M.S., Forteza I., Rosenberg G., Haygood M.G., Schmidt E.W, & Concepcion G.P. (2020). Synergistic anti-methicillin-resistant Staphylococcus aureus (MRSA) activity and absolute stereochemistry of 7,8-dideoxygriseorhodin C. The Journal of Antibiotics, 73(5), 290-298.