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Qstar xl hybrid lc ms ms system

Manufactured by Thermo Fisher Scientific
Sourced in United States

The QSTAR® XL Hybrid LC/MS/MS System is a liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform designed for high-performance analytical applications. It combines the advantages of quadrupole and time-of-flight (TOF) mass analyzers to provide accurate mass and high-resolution capabilities for a wide range of analytes.

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4 protocols using qstar xl hybrid lc ms ms system

1

Mass Spectroscopy Procedure for Resuspended Samples

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Example 10

Mass Spectroscopy

Samples were resuspended in carrier solution (50% water, 50% methanol and 0.01% formic acid). Mass spectroscopy was performed using a high-performance, hybrid quadrupole time-of-flight mass spectrometer—QSTAR® XL Hybrid LC/MS/MS System (Applied Bio systems, Foster City, Calif., USA)—in positive ion mode, at room temperature, with an IonSpray source for API 150EX™, API 3000™ and QSTAR® Systems (Applied Biosystems). Data were analyzed using Analyst QS version 1.1.

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2

Analytical Techniques for Structural Elucidation

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UV–Vis spectra were obtained by using a Shimadzu Prominence HPLC System (Shimadzu, Kyoto, Japan) coupled with a PDA detector. High-resolution electrospray ionization mass spectra were gathered using a QSTAR® XL Hybrid LC/MS/MS System (Applied Biosystems, Foster City, CA, USA) equipped with turbo ion spray source, delivering the sample at a rate of 40 µl min−1. NMR data were collected using a Varian 500-MHz NMR spectrometer with 5 mm Varian Oneprobe (1H 500 MHz, 13C 125 MHz). Residual signals from solvents were used for referencing. Semipreparative HPLC was performed using a Phenomenex C18 column (Luna 250 × 10 mm, 5 µm, 4.0 ml min−1). ECD spectra were obtained on an Aviv Biomedical Model 410 CD Spectrometer.
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3

Mass Spectrometry Protocol for Metabolite Analysis

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Example 10

Samples were resuspended in carrier solution (50% water, 50% methanol and 0.01% formic acid). Mass spectroscopy was performed using a high-performance, hybrid quadrupole time-of-flight mass spectrometer—QSTAR® XL Hybrid LC/MS/MS System (Applied Bio systems, Foster City, Calif., USA)—in positive ion mode, at room temperature, with an IonSpray source for API 150EX™, API 3000™ and QSTAR® Systems (Applied Biosystems). Data were analyzed using Analyst QS version 1.1.

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4

Comprehensive Analytical Characterization of Compounds

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UV-vis spectra were obtained using a Shimadzu Prominence HPLC System (Shimadzu, Kyoto, Japan) coupled with a PDA detector. High-resolution electrospray ionization mass spectra were gathered using a QSTAR® XL Hybrid LC/MS/MS System (Applied Biosystems, Foster City, CA, USA) equipped with turbo ion spray source, delivering the sample at a rate of 40 μl/min. NMR data were collected using a Varian 500 MHz NMR spectrometer with 5 mm Varian Oneprobe (1H 500 MHz, 13C 125 MHz). Residual signals from solvents were used for referencing. Semi-preparative HPLC was performed using a Phenomenex C18 column (Luna 250 X 10 mm, 5 μm, 4.0 ml/min). ECD spectra were obtained on an Aviv Biomedical Model 410 CD Spectrometer.
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