Tissue sections were deparaffinized in xylene and then rehydrated sequentially in 100, 90, and 70% ethanol in distilled water. The slides were then incubated in fresh hematoxylin (Invitrogen Corporation, Frederick, MD, USA) for 20 min, washed in distilled water, incubated in acidified eosin solution (Richard-Allan Scientific, Kalamazoo, MI, USA) for 4 min and then washed in distilled water. Finally, the slides were dehydrated in 90 and 100% ethanol, air dried and mounted24 .
Acidified eosin solution
Manufactured by Thermo Fisher Scientific
Sourced in United States
Acidified eosin solution is a laboratory reagent used in various staining and analytical procedures. It is a prepared solution containing eosin, a red fluorescent dye, which is acidified to maintain a specific pH. This solution is commonly used in histology, cytology, and other fields of microscopy to stain biological samples for visualization and identification purposes.
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Lab products found in correlation
2 protocols using acidified eosin solution
1
Hematoxylin and Eosin Staining of Tissue Sections
2
Tissue Staining with Hematoxylin and Eosin
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We deparaffinized tissue sections by heating for 1 hour at 60°C and immersion in xylene for 30 min. We sequentially rehydrated tissues by 99, 96, and 70% ethanol and isopropanol. We immersed the sections in hematoxylin (Invitrogen Corporation, Frederick, MD, USA) for 20 min, followed by washing with distilled water and immersion in acidified eosin solution (Richard-Allan Scientific, Kalamazoo, MI, USA) for 4 min. After the final wash with distilled water, we sequentially dehydrated sections through immersion in 96 and 99% ethanol and xylene, followed by mounting with the tissue-mounting medium Pertex (Medite GmbH, Burgdorf, Switzerland).
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