The microcapsules were ground and mixed with spectroscopic grade potassium bromide. The mixture was pressed (7 Kgf for 7 min) to result in a compact and clear disc, and was analyzed in the FT-IR Spectrophotometer Frontier (Perkin Elmer, Shelton, CT, USA) in the region of 4000–400 cm−1, with a resolution of 4 cm−1 and 32 accumulated scans.
Frontier ft ir spectrophotometer
Manufactured by PerkinElmer
Sourced in United States
The Frontier FT-IR spectrophotometer is a versatile laboratory instrument designed for infrared spectroscopy analysis. It utilizes Fourier transform infrared (FT-IR) technology to capture and interpret infrared radiation absorption and emission spectra of various samples. The core function of the Frontier FT-IR is to provide precise and reliable infrared spectroscopic data for identification, characterization, and quantification of chemical compounds and materials.
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23 protocols using frontier ft ir spectrophotometer
1
FT-IR Spectroscopic Analysis of Microcapsules
2
Spectroscopic Characterization of Organic Compounds
3
Characterization of Synthetic Compounds
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FT-IR spectrum : The FT-IR spectrum was collected using a PerkinElmer Frontier FT-IR spectrophotometer. The synthesized crystal compounds were powdered with potassium bromide, and the diffuse reflectance technique was used to capture the spectra; 1H NMR spectrum : The proton NMR spectrum was captured using a Bruker 400 MHz NMR spectrometer adjusted at 400 MHz. To make the samples, dissolve 20 mg of the synthesized compound in 0.5 mL of CDCl3 containing 0.03 percent TMS; 13C NMR spectrum : Proton decoupled 13C NMR spectra were captured using a Bruker 400 MHz spectrometer set to 100 MHz. 13C NMR Spectra were measured using solutions made by dissolving 20 mg of the substance in 0.5 mL of CDCl3 adding a few drops of TMS as an internal reference: Mass spectrum : A low-resolution mass spectrum was recorded to use an Agilent 6130B Quadrupole LC/MS in ESI mode with an Agilent 1260 Infinity LC system. Thermo Scientific Hypersil 150 × 2.1 mm 5-micron column was used to analyze the all samples.
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4
Chromatography, HPLC, and Spectroscopic Analysis
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The column chromatography made use of silica gel (SiO2, 200−300 mesh, Qingdao Marine Chemical Inc., Qingdao, China). Preparative HPLC was performed using a Shimadzu LC-20AT HPLC pump (Shimadzu Corporation, Nakagyo−ku, Kyoto, Japan) and installed with an SPD-20A dual λ absorbance detector (Shimadzu Corporation, Nakagyo−ku, Kyoto, Japan) and a Capcell−Pak C18 UG80 HPLC column (250 mm×20 mm, Shiseido Co., Ltd., Minato-ku, Tokyo, Japan) and a Spolar HPLC packed column (250 mm × 4.6 mm, Shiseido Co., Ltd., Minato-ku, Tokyo, Japan). The melting point used the melting point apparatus WRS-3 (Shenguang, Shanghai, China) to record. UV data were obtained on a Shimadzu UV-Vis-NIR spectrophotometer (Shimadzu Corporation, Nakagyo-ku, Kyoto, Japan). IR spectra were recorded on a PerkinElmer Frontier FT-IR spectrophotometer (PerkinElmer Inc., Waltham, MA, USA). The 1D and 2D NMR experiments were measured with Bruker Avance 400 spectrometer and Bruker Avance 600 spectrometer (Bruker Bio Spin AG, Industriestrasse 26, Fällanden, Switzerland). The chemical shifts were relative to the residual solvent signals (CDCl3: δH 7.260 and δC 77.000; acetone-d6: δH 2.050 and δC 29.840; and methanol-d4: δH 3.310 and δC 49.000). HR-ESI-MS data were collected on a Thermo Fisher LTQ Orbitrap Elite high-resolution mass spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA).
5
Fourier Transform Infrared Spectroscopic Analysis
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Fourier transform infrared spectroscopy (FTIR) measurements were made on a Frontier FTIR spectrophotometer (Perkin Elmer, Waltham, MA, USA), coupled to a universal attenuated total reflectance (ATR) sampling accessory equipped with a zinc selenide measurement cell (Perkin Elmer, Waltham, MA, USA). All fiber samples (untreated and biologically treated) were dried for 48 h in a vacuum oven at a temperature of 50 °C, and then stored in a vacuum desiccator at room temperature. Then, the samples were placed directly on the ATR sampling accessory window crystal and scanned at a range of 4000 cm−1 to 650 cm−1, with a resolution of 8 cm−1, and an average of 5 accumulations.
6
Characterization of SRC and RC Film Samples
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The X-ray diffraction (XRD) patterns of the SRC and RC film samples were measured using a benchtop X-ray diffractometer (Miniflex 600, Rigaku, Wilmington, MA, USA). For each measurement, a rectangular piece of film was mounted on a sample glass slide and the diffraction spectrum was recorded using Cu Kα radiation (λ = 0.154 nm) at 40 kV and 20 mA over the 3–40° range. The Fourier-transform infrared (FTIR) spectra of the film samples were obtained using a Perkin-Elmer Frontier FTIR spectrophotometer coupled with a diamond crystal attenuated total reflectance accessory (Perkin Elmer, Waltham, MA, USA) over the wavenumber range 4000 to 600 cm−1 with 64 scans averaged for each sample.
7
Synthesis and Characterization of Novel Compounds
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All oof the chemicals required for the synthesis of intermediate as well as target compounds were procured from Sigma-Aldrich (Munich, Germany) and Samchun Chemicals (Daejeon, South Korea) and used without purification. 13C NMR and 1H NMR spectra were taken in DMSO-d6 using a Bruker Avance Ⅱ (Germany) NMR spectrophotometer at 126 and 500 MHz, respectively. The mass analysis (LC-MS) was recorded on a 2795/ZQ2000 (waters) spectrometer. The IR spectra were recorded on a Frontier FTIR spectrophotometer (PerkinElmer, Greenville, SC, USA). The progress of the chemical reactions was scrutinized by a thin layer chromatography (TLC) system. The physical factors (such as melting points) of the nine compounds denoted as 9(a–i) were determined by using Fisher Scientific (Waltham, MA 02451, USA) melting point apparatus and were uncorrected. The coupling constant and chemical shift values were measured in ppm and Hz, respectively. Electrospray ionization (ESI) was used to produce ions for FTIR, LC-MS, 1H NMR, and 13C NMR spectral studies.
8
ATR-FTIR Analysis of Aqueous Samples
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The FTIR spectra were recorded using a universal ATR sampling accessory on a Frontier FTIR spectrophotometer (PerkinElmer). Prior to scanning, the FTIR spectrophotometer was blanked with ultrapure water. Samples were prepared at 10 mM and 1× PBS with 4 µl spotted onto the ATR accessory. Each sample was scanned 50 times with the average of the spectra reported.
9
Spectroscopic and Mass Spectrometric Analysis
10
Bio-Synthesis of Gold Nanoparticles from Bee Pollen
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A digital microscopic image of bee pollen was obtained with the help of the Trinocular Stereomicroscope (SMZ745T, Nikon, Japan). The biosynthesis of AuNPs and its catalytic activity was determined by visual inspection and checking the absorption spectra using UV-visible spectrophotometer (Thermo Spectronic, GENESYS™ 8, England) and (SPECORD ® S600 from Analytik Jena, Germany). The shape/size and selective area electron diffraction (SAED) pattern of AuNPs are studied by placing a drop of AuNPs on a carbon coated copper disc and visualizing it using high resolution transmission electron microscopy, HR-TEM (FEI, TECNAI, G2 spirit twin, Holland) having 80 kV accelerating voltage. The average particle diameter and Dynamic Light Scattering (DLS) of the AuNPs was determined using the HORIBA, DLS version LB-550 program (Japan). The Fourier transform infrared spectroscopy (FTIR) measurements of pollen and AuNPs in the attenuated total reflectance (ATR) mode were recorded using Frontier FT-IR spectrophotometer (Perkin Elmer, USA) to identify bee pollen constituents responsible for synthesis and capping of AuNPs. The UV-vis and FTIR graphics have been drawn on the OriginPro 8 program. The geometry of AuNPs was examined using X-ray diffraction (XRD) analyses (PANalytical, Malvern copper λ = 1.54059 Å and EMPYREAN diffractometer, UK) at 45 kV and 40 mA.
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