Lung adenocarcinoma cell lines including A549 (CM-0016) and H1299 (CM-0165) were purchased from Procell (Wuhan, China). Both cell lines were authenticated by short tandem repeat (STR) profiling. A549 cells were cultured in Ham’s F-12K medium (PM150910, Procell) supplemented with 10% FBS (164210-500, Procell) and 1% P/S (PB180120, Procell). H1299 cells were cultured in RPMI-1640 medium (PM150110, Procell) plus 10% FBS (164210-500, Procell) and 1% P/S(PB180120, Procell). All cell lines were routinely maintained at 37 °C in a 5% CO2 incubator.
Pb180120
Manufactured by Procell
Sourced in China, United States
The PB180120 is a laboratory equipment designed for general use in scientific research and analysis. It serves as a versatile tool to facilitate various experimental and testing procedures. The product's core function is to provide a reliable and standardized platform for conducting laboratory-based activities.
Automatically generated - may contain errors
Lab products found in correlation
Pm150210, by Procell (10 mentions)
Pm150110, by Procell (9 mentions)
Pm150410, by Procell (5 mentions)
Pm150910, by Procell (3 mentions)
Hct116, by Procell (3 mentions)
Fetal bovine serum (fbs), by Procell (3 mentions)
Mda mb 231, by American Type Culture Collection (3 mentions)
Pm150312, by Procell (3 mentions)
H1299, by Procell (2 mentions)
Rpmi 1640 medium, by Procell (2 mentions)
Sum 149, by BasalMedia (2 mentions)
Sum149, by American Type Culture Collection (2 mentions)
Ham s f12 medium, by BasalMedia (2 mentions)
Mcf 10a, by American Type Culture Collection (2 mentions)
Mcf 7, by American Type Culture Collection (2 mentions)
Mda mb 231, by Procell (2 mentions)
Rpmi 1640, by Procell (2 mentions)
Pm150810, by Procell (2 mentions)
Dulbecco modified eagle medium (dmem), by Procell (2 mentions)
Ham s f12k, by Procell (2 mentions)
53 protocols using pb180120
1
Lung Adenocarcinoma Cell Line Cultivation
2
Laparoscopic Hemicolectomy for Colon Cancer
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The pathology specimens were acquired from the biospecimen repository of Xinqiao Hospital. The 93 patients from our Department of General Surgery, Xinqiao Hospital, all underwent laparoscopic left (or right) hemicolectomy during 2015–2016. All patients signed an informed consent upon admission to our hospital. The application for surgical specimens was approved by the Clinical Ethics Committee of the Second Affiliated Hospital of Army Medical University of the PLA (no. 2022-036-01). The study methodologies strictly conformed to the standards set by the Declaration of Helsinki.
Colon cancer cell lines, including SW620 and HCT116, were purchased from Procell Life Science & Technology Co., Ltd. Then, they were cultured in the corresponding complete medium, which was Leibovitz’s L-15 (PM151010)+10% FBS (164210-500)+1% P/S (PB180120) or MEM+10% FBS (164210-500)+1% P/S (PB180120), in an incubator of 37°C constant temperature with 5% CO2.
Colon cancer cell lines, including SW620 and HCT116, were purchased from Procell Life Science & Technology Co., Ltd. Then, they were cultured in the corresponding complete medium, which was Leibovitz’s L-15 (PM151010)+10% FBS (164210-500)+1% P/S (PB180120) or MEM+10% FBS (164210-500)+1% P/S (PB180120), in an incubator of 37°C constant temperature with 5% CO2.
3
Culturing Primary Podocytes and Mesangial Cells
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Human primary podocytes were purchased from Huatuobo (HTX2426, OTWO, Shenzhen, China) and cultured in RPMI1640 (PM150110, Procell, Wuhan, China) supplemented with 20 µ/ml γ-interferon, 10% foetal bovine serum (164210-500, Procell), and 1% penicillin/streptomycin (PB180120, Procell) at 33°C in a 5% CO2-containing environment. The cells were further cultured at 37°C for 10 days to induce podocyte maturation.
HMCs derived from human glomerular mesangial cells were purchased from Procell (CL-0619) and cultured in Dulbecco’s Modified Eagle Medium (Procell, PM150210) supplemented with 10% foetal bovine serum (164210-500, Procell, China) and 1% penicillin/streptomycin (PB180120, Procell). The cells were subcultured at 37°C in a 5% CO2 atmosphere.
HMCs derived from human glomerular mesangial cells were purchased from Procell (CL-0619) and cultured in Dulbecco’s Modified Eagle Medium (Procell, PM150210) supplemented with 10% foetal bovine serum (164210-500, Procell, China) and 1% penicillin/streptomycin (PB180120, Procell). The cells were subcultured at 37°C in a 5% CO2 atmosphere.
4
Cell Line Cultivation and Validation
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The 786-O (CL-0010), ACHN (CL-0021) human renal cancer cell lines, and 293T cells (CL-0005) were purchased from Procell Life Science & Technology (Wuhan, China). All cells were subjected to STR authentication by Procell Life Science & Technology and tested for mycoplasma contamination. The 786-O cells were cultured with RPMI-1640 (PM150110, Procell Life Science & Technology) plus 10% fetal bovine serum (FBS) (164210-500, Procell Life Science & Technology) and 1% penicillin/streptomycin (P/S) (PB180120, Procell Life Science & Technology). The ACHN cells were maintained with MEM (PM150410, Procell Life Science & Technology) supplemented with 10% FBS (164210-500, Procell Life Science & Technology) and 1% P/S (PB180120, Procell Life Science & Technology). The 293T cells were cultured in DMEM (PM150210, Procell Life Science & Technology) supplemented with 10% FBS and 1% P/S. All of these cells were maintained in 5% CO2 at 37 °C.
5
Cell Line Culture Protocols
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Normal laryngeal cell line HuLa‐PC and LC cell line (AMC‐HN‐8, SNU‐46, SNU‐899, and SNU‐1076) bought from Fenghuishengwu (China) were incubated in RPMI‐1640 medium (PM150110, Procell Life Science&Technology) enriched with 1% penicillin–streptomycin (PB180120, Procell) and 10% fetal bovine serum (FBS; 164,210, Procell) at 37°C in a humidified atmosphere containing 5% CO2.
6
HK2 Cells Respond to ELA and Aldosterone
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The human renal tubular duct epithelial cells HK2 were purchased from the Procell Life Science & Technology Company, Wuhan, China (CL-0109). The HK2 cells were grown in Dulbecco’s modified Eagle Medium F12 (Gibco, C11330500BT) supplemented with 10% (V/V) fetal bovine serum (HyClone, SV30160.03) and 1% antibiotics (Procell, PB180120) in tissue culture flasks. The cells were pretreated for 1 h with ELA peptide (1 nM, IGE Biotech, Guangzhou, China) followed by Aldosterone (0.1 µM, Sigma, A9477) treatment. After 24 h, cells were harvested for the analysis of gene expression, ROS production, and NADP+/NADPH.
7
Culturing Human Thyroid Cancer Cells
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Human thyroid cancer cell lines IHH-4 (JCRB1079) and CAL-62 (CL-0618) were bought from JCRB Cell Bank (Tokyo, Japan) and Procell (Wuhan, China), respectively. Both of cells were cultured in the DMEM medium (PM150210, Procell) with 10% fetal bovine serum (FBS, 164210–500, Procell) and 1% streptomycin-penicillin (P/S, PB180120, Procell) at 37°C with 5% CO2.
8
Comprehensive Kidney Cancer Cell Lines
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All patient tissue samples were obtained from the Department of Urology, Peking University First Hospital after signing informed consent. Pathological results were diagnosed by at least two senior pathologists from Peking University Institute of Urology. The HK2, OSRC2, 786O, 769P, CAKI2, A498, ACHN, CAKI1, THP1 and HUVECs cells were purchased from the American Type Cultrue Collection (ATCC). All cells were cultured in DMEM (Gibico 11965118) or Endothelial Cell Medium (ScienCell 34,469) with 10% fetal bovine serum (VivaCell 2204031) and 1% penicillin-streptomycin (Procell PB180120). All cells were cultured in an incubator at 37℃ with 5% CO2.
9
Cultivation of Colorectal Cancer Cell Lines
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Human colorectal cancer cell lines SW480 (CL-0223) and HCT116 (CL-0096) were purchased from Procell (Wuhan, China). SW480 cells were grown in Leibovitz’s L-15 medium (PM151010, Procell) containing 10% fetal bovine serum (FBS; 164210–500, Procell) and 1% penicillin-streptomycin solution (P/S; PB180120, Procell), and HCT116 cells were cultured in McCoy’s 5A medium (PM150710, Procell) supplemented with 10% FBS and 1% P/S. All cells were incubated under the Heracell 240i CO2 Incubator (Thermo Scientific, Waltham, Massachusetts, USA).
10
Osteogenic Potential of MC3T3-E1 Cells
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Mouse embryonic osteoblast MC3T3-E1 cells were cultured in Dulbecco’s modified eagle’s medium (DMEML31600-500, Solarbio TECH, Beijing, China), supplemented with 1% penicillin-streptomycin solution (PB180120, Procell Life Science & Technology Co., Ltd, Wuhan, China). The cells were cultured in humidified with 5% CO2 at 37 °C. All samples were sterilised in an autoclave for 0.5 h before experiments. After incubation for 1–28 d, cell adhesion and proliferation were detected using a CCK-8 assay, and the osteogenesis was examined through alkaline phosphatase (ALP) activity assay and alizarin red S (ARS).
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