3i marianas

Manufactured by Zeiss

The 3i Marianas is a high-performance, modular microscope system designed for advanced imaging applications. It features a stable and customizable platform that supports a wide range of microscopy techniques, including confocal, widefield, and multi-modal imaging. The 3i Marianas is engineered to deliver reliable and consistent performance for demanding research and industrial applications.

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Lab products found in correlation

Csu x1, by Nikon (1 mentions) Axiovert 200m inverted microscope, by Zeiss (1 mentions) Csu w1, by Zeiss (1 mentions) P35g 1.5 14 c, by MatTek (1 mentions)

Spelling variants of this product

3i Marianas system (3 citations) 3i Marianas spinning disk confocal microscope (2 citations)

3 protocols using 3i marianas

Confocal Imaging of Fixed Intestine Tissues

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Images of fixed tissue were taken either with a Yokogawa CSU-W1/Zeiss 3i Marianas spinning disk confocal microscopy system or a Andor CSU-X1/Nikon Ti-E spinning disk confocal microscopy system. Intestines were imaged using a ×40 PlanFleur objective or, for gut size measurements, ×10 PlanFleur objective. Images were analyzed and processed using ImageJ (NIH, Bethesda, MD) and Adobe Photoshop. Figures were composed in Adobe Illustrator. Except for quantification of PH3+ cells and gut length, which was performed in the entire gut, only the R4 region of the posterior midgut was analyzed for consistency.

Hu D.J., Yun J., Elstrott J, & Jasper H. (2021). Non-canonical Wnt signaling promotes directed migration of intestinal stem cells to sites of injury. Nature Communications, 12, 7150.

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Microscopic Visualization of AZN-D1 Binding

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CHO/DC-SIGN cells were cultured on gelatin coated glass slides. AZN-D1 coated beads were added to the cells and followed for different time points. Cells were analyzed by means of a 3I Marianas™ digital imaging microscopy workstation (Zeiss Axiovert 200 M inverted microscope Carl Zeiss), equipped with a nanostepper motor (Z-axis increments 10 nm) and a cooled CCD camera (Cooke Sensicam, 1,280 × 1,024 pixels Cooke Co). Visualization was performed with a 40× air lens. The microscope, camera, and data viewing process were controlled by SlideBook™ software (version 4.0.8.1 Intelligent Imaging Innovations).

Horrevorts S.K., Duinkerken S., Bloem K., Secades P., Kalay H., Musters R.J., van Vliet S.J., García-Vallejo J.J, & van Kooyk Y. (2018). Toll-Like Receptor 4 Triggering Promotes Cytosolic Routing of DC-SIGN-Targeted Antigens for Presentation on MHC Class I. Frontiers in Immunology, 9, 1231.

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Long-term live imaging of Drosophila intestine

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Intestines from adult female flies were dissected in culture media containing 2mM CaCl₂, 5mM KCl, 5mM HEPES, 8.2mM MgCl₂, 108mM NaCl, 4mM NaHCO₃, NaH₂PO₄,10mM sucrose, 5mM trehalose, and 2% fetal bovine serum (Adult Hemolymph-like Saline, AHLS). Intestines were transferred to a 35mm glass bottom dish (MatTek, P35G-1.5-14-C), embedded in 4% low melting agarose (in AHLS), and submerged in AHLS. The posterior midgut was imaged either at intervals of 10 min for two hours or 10-15 min for 10-15 hours (long-term lineage tracing) on a Yokogawa CSU-W1/Zeiss 3i Marianas spinning disk confocal microscopy system. For the long-term lineage tracing experiments, the 568 channel was not imaged for up to two hours to reduce the effects of any possible phototoxicity.

Hu D.J, & Jasper H. (2019). Control of Intestinal Cell Fate by Dynamic Mitotic Spindle Repositioning Influences Epithelial Homeostasis and Longevity. Cell reports, 28(11), 2807-2823.e5.

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