Anti ha ab18181

Manufactured by Abcam

Sourced in United States

Anti-HA (ab18181) is a mouse monoclonal antibody that recognizes the Hemagglutinin (HA) tag. The HA tag is a commonly used epitope tag that can be fused to the N- or C-terminus of a protein to facilitate its detection and purification. Anti-HA (ab18181) can be used to detect and immunoprecipitate HA-tagged proteins in a variety of applications, such as Western blotting, immunoprecipitation, and immunocytochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (4 mentions) Anti lc3a b 4108, by Cell Signaling Technology (2 mentions) Dulbecco s phosphate buffered saline dpbs d8537, by Merck Group (2 mentions) Anti flag sab4200071, by Merck Group (1 mentions) Anti gfp, by Abmart (1 mentions) Anti gapdh ab8245, by Abcam (1 mentions) Anti vimentin 5741, by Cell Signaling Technology (1 mentions) Anti gfp 50430 2 ap, by Proteintech (1 mentions) Anti puromycin 3rh11, by Kerafast (1 mentions) Anti flag f1804, by Merck Group (1 mentions) Anti ha ab9110, by Abcam (1 mentions) Alexa fluor 488 anti mouse a11029, by Thermo Fisher Scientific (1 mentions)

4 protocols using anti ha ab18181

Molecular Profiling of Cellular Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-Myc (2276), anti-GAPDH (2118), and anti-LC3A/B (4108) were purchased from Cell Signaling Technology. Anti-HA (ab18181) was purchased from Abcam. Flag (SAB4200071), lipopolysaccharide (LPS; serotype 0128: B12), chloroquine (CQ; C6628), dimethyl sulfoxide (DMSO; 472301), puromycin (P8833), paraformaldehyde (P6148), Triton X-100 (T8787), 3-methyladenine (3-MA; M9281), gentamicin (G1272), deoxycholate (D6750), and Dulbecco's phosphate-buffered saline (DPBS; D8537) were purchased from Sigma-Aldrich. Lipofectamine 2000 (11668019) was purchased from Thermo Fisher Scientific.

Kim M.J., Choi B., Kim J.Y., Min Y., Kwon D.H., Son J., Lee J.S., Lee J.S., Chun E, & Lee K.Y. (2021). USP8 regulates liver cancer progression via the inhibition of TRAF6-mediated signal for NF-κB activation and autophagy induction by TLR4. Translational Oncology, 15(1), 101250.

+ Open protocol

+ Expand

Antibody Validation for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-MYC (2276), anti-GAPDH (2118), and anti-LC3A/B (4108) were purchased from Cell Signaling Technology. Anti-FLAG (SAB4200071) was purchased from Sigma-Aldrich. Anti-HA (ab18181) and anti-USP15 (ab97533) were purchased from Abcam. Lipopolysaccharide (LPS; serotype 0128: B12), chloroquine (CQ; C6628), dimethyl sulfoxide (DMSO; 472301), puromycin (P8833), paraformaldehyde (P6148), Triton X-100 (T8787), 3-methyladenine (3-MA; M9281), gentamicin (G1272), deoxycholate (D6750), and Dulbecco’s phosphate-buffered saline (DPBS; D8537) were purchased from Sigma-Aldrich. Lipofectamine 2000 (11668019) was purchased from Thermo Fisher Scientific.

Kim M.J., Min Y., Jeong S.K., Son J., Kim J.Y., Lee J.S., Kim D.H., Lee J.S., Chun E, & Lee K.Y. (2022). USP15 negatively regulates lung cancer progression through the TRAF6-BECN1 signaling axis for autophagy induction. Cell Death & Disease, 13(4), 348.

+ Open protocol

+ Expand

Antibody Reagents for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-Flag (F3165), anti-PARP12 (HPA003584), and anti-FHL2 (HPA006028) antibodies were purchased from Sigma. Anti-HA (ab18181) and anti-GAPDH (ab8245) antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-snail (3879S), anti-N-cadhenrin (14215S), and anti-vimentin (5741S) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-GST, anti-His, and anti-GFP antibodies were obtained from Abmart. Anti-(ADP-ribose) antibody (A01316) was purchased from GenScript
Glutathione Sepharose and Ni Sepharose were purchased from GE Heathcare (Little Chalfont, UK, EU). High-capacity Streptavidin Agarose (20359) and S-protein Agarose (69704) were bought from Thermo Scientific and Novagen, respectively.

Shao C., Qiu Y., Liu J., Feng H., Shen S., Saiyin H., Yu W., Wei Y., Yu L., Su W, & Wu J. (2018). PARP12 (ARTD12) suppresses hepatocellular carcinoma metastasis through interacting with FHL2 and regulating its stability. Cell Death & Disease, 9(9), 856.

+ Open protocol

+ Expand

Antibodies Used in Protein Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following commercially available antibodies were used in this paper: Anti-HA ab9110 (Abcam), Anti-HA ab18181 (Abcam), Anti-Flag F1804 (Sigma), Anti-Huntingtin Mab2166 (Chemicon, Sigma), Anti-GFP 50430-2-AP (Proteintech), Anti-RFP R10367 (Life Technologies), Anti α-Tubulin 66031–1 (Proteintech), Anti-FAS II-1D4 (DSHB), Anti-Repo 8D12 (DSHB), Anti-Puromycin-3RH11(Kerafast), Anti-Ref2P-ab178440 (Abcam), Anti-HA epitope tag clone 16B12 (Biolegend), Multi Ubiquitin chain monoclonal-cloneFK2 14220 (Cayman), Alexa flour 488 Phalloidin-A12379 (Life Technologies), Alexa flour 555 phalloidin-A34055 (Life Technologies), Alexa Fluor 555 anti-mouse A21424 (Life Technologies), Alexa Fluor 488 anti-mouse A11029 (Life Technologies), Alexa Fluor 555 anti-Rabbit A11034 (Life Technologies), Alexa Fluor 488 anti-Rabbit A21429 (Life Technologies).
Anti-Orb2 and anti-Mrj antibodies were raised against recombinant 6X Histidine-tagged full-length protein, in rabbit and guinea pig for Orb2 and guinea pig for Mrj. For immunoprecipitation, protein-A agarose beads (Repligen 102500–03) and RFP trap magnetic beads (Chromo Tech rtma) were used.

Desai M., H.e.m.a.n.t., Deo A., Naik J., Dhamale P., Kshirsagar A., Bose T, & Majumdar A. (2024). Mrj is a chaperone of the Hsp40 family that regulates Orb2 oligomerization and long-term memory in Drosophila. PLOS Biology, 22(4), e3002585.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!