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Metamorph imaging 7

Manufactured by Molecular Devices
Sourced in United States

Metamorph Imaging 7.0 software is a comprehensive image acquisition and analysis platform designed for life science research. It provides a suite of tools for capturing, processing, and analyzing images from a variety of microscopy techniques.

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2 protocols using metamorph imaging 7

1

Vascular Morphometric Analysis Post-Injury

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The whole artery was cut in 6 µm serial sections, and six cross-sections from regular intervals throughout each artery were stained with haematoxylin–eosin. For morphometric analyses, Metamorph Imaging 7.0 software (Molecular Devices) was used to measure external elastic lamina, internal elastic lamina, and lumen circumference as well as medial and neointimal area 21 days after injury.
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2

Quantifying Femoral Artery Re-endothelialization

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Mice were injected with 0.5 mL of 0.5% Evans blue dye (Sigma-Aldrich, Munich, Germany) in PBS 10 days after vascular injury, as previously described.14 (link) Mice were killed 15 min after injection of Evans blue dye and then perfused with 4% PFA via the left ventricle. The injured femoral artery was excised and opened longitudinally to expose the luminal surface. Planimetric analysis was performed by Metamorph Imaging 7.0 software (Molecular Devices, Downingtown, PA, USA) and the ratio of re-endothelialized area (defined as area not stained with Evans blue/total injured surface area) was determined.
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