Cells (2.0 × 106 cells in 100 mm dish) were treated with different concentrations of Elental (0, 0.1, 0.5, 1, 5, 10, 50, and 100 μg/mL), which was dissolved in DMEM/Ham’s F-12 medium without FBS. The cells were lysed with RIPA Buffer (Thermo Fisher Scientific). Whole cell lysates were subjected to electrophoresis on 10% sodium dodecyl sulfate–polyacrylamide gels (Thermo Fisher Scientific), and then transferred to a polyvinylidene difluoride membrane (Thermo Fisher Scientific). After blocking, the membranes were incubated with the anti-fibroblast growth factor 2 (FGF2) rabbit polyclonal antibody (Santa Cruz Biotechnology, Inc, Santa Cruz, CA, USA) or anti-α-tubulin monoclonal antibody (Santa Cruz Biotech) followed by Novex alkaline-phosphatase conjugated (goat) anti-rabbit or (goat) anti-mouse immunoglobulin G (IgG) secondary antibody (Thermo Fisher Scientific). The antibodies were detected using a chromogenic immunodetection system, WesternBreeze (Thermo Fisher Scientific) according to the manufacturer’s instructions.
Novex alkaline phosphatase conjugated goat anti rabbit or goat anti mouse immunoglobulin g igg secondary antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Novex alkaline-phosphatase conjugated (goat) anti-rabbit or (goat) anti-mouse immunoglobulin G (IgG) secondary antibody is a laboratory reagent used to detect the presence of rabbit or mouse primary antibodies in various immunoassay techniques. The secondary antibody is conjugated with the enzyme alkaline phosphatase, which catalyzes a colorimetric reaction upon the addition of a suitable substrate, allowing for the visual detection and quantification of the target primary antibody.
Automatically generated - may contain errors
Lab products found in correlation
Anti α tubulin monoclonal antibody, by Santa Cruz Biotechnology (2 mentions)
Western breeze, by Thermo Fisher Scientific (2 mentions)
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Polyvinylidene difluoride membrane, by Thermo Fisher Scientific (1 mentions)
Sodium dodecyl sulfate polyacrylamide gel, by Thermo Fisher Scientific (1 mentions)
Radio immunoprecipitation assay ripa buffer, by Thermo Fisher Scientific (1 mentions)
Sds polyacrylamide gel, by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
2 protocols using novex alkaline phosphatase conjugated goat anti rabbit or goat anti mouse immunoglobulin g igg secondary antibody
1
Elental Modulates FGF2 Protein Levels
2
Western blot analysis of HCAM and CD13
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Whole cell lysates were prepared using Radioimmunoprecipitation assay (RIPA) buffer (Thermo Fisher scientific) and were subjected to electrophoresis on 10 % SDS-polyacrylamide gels (Thermo Fisher scientific), and then transferred to a PVDF membrane (Thermo Fisher scientific). After blocking, the membranes were incubated with the anti-HCAM (CD44) mouse monoclonal antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and anti-CD13 rabbit monoclonal antibody (Epitomics Inc., Burlingame, CA, USA) followed by Novex® alkaline-phosphatase conjugated (goat) anti-rabbit or (goat) anti-mouse immunoglobulin G (IgG) secondary antibody (Thermo Fisher scientific). The antibodies were detected using a chromogenic immunodetection system, WesternBreeze (Thermo Fisher scientific) according to the manufacturer's instructions. Also, anti- α- tubulin monoclonal antibody (Santa Cruz Biotech.) was used for normalization of Western blot analysis.
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