To validate the interaction between ZFAS1 and miR-647, cells were transfected with pCMV-MS2, pCMV-ZFAS1-MS2, pCMV-ZFAS1-mut-MS2 and pCMV-FLAG-MS2. Forty-eight hours later, cells were used to perform RIP assay using 5 μg anti-FLAG antibody (Sigma) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, Bedford, MA) according to the manufacturer’s instructions. To detect the interaction between METTL3 or AGO2 and ZFAS1, RIP assay was carried out by using 5 μg anti-METTL3 antibody (Abcam) or anti-AGO2 antibody (Abcam) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit. IgG was taken as a negative control. The RNA fraction isolated and purified by RIP assay was then examined by RT-PCR.
Magna rip rna binding protein immunoprecipitation kit
Manufactured by Abcam
Sourced in China
The Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit is a tool for the immunoprecipitation of RNA-binding proteins and their associated RNAs from cell or tissue lysates.
Automatically generated - may contain errors
Lab products found in correlation
Magna rip rna binding protein immunoprecipitation kit, by Merck Group (2 mentions)
Anti mettl3 antibody, by Abcam (1 mentions)
Anti ago2 antibody, by Abcam (1 mentions)
Anti flag antibody, by Merck Group (1 mentions)
Gfp antibody, by Roche (1 mentions)
Drosha antibody, by Cell Signaling Technology (1 mentions)
Pms2 gfp, by Addgene (1 mentions)
2 protocols using magna rip rna binding protein immunoprecipitation kit
1
ZFAS1 Interaction with miR-647 and Associated Proteins
2
RIP Assay for SPINT1-AS1 Interactions
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pcDNA3.1-MS2, pcDNA3.1-MS2-SPINT1-AS1, or pcDNA3.1-MS2-SPINT1-AS1-del was co-transfected with pMS2-GFP (Addgene) into HeLa cells. After culture for another 48 h, cells were used to conduct RIP assays using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica, MA, United States) and a GFP antibody (5 μg per reaction; 11814460001, Roche). For anti-DROSHA, anti-DGCR8 RIP, HeLa cells with SPINT1-AS1 or SPINT-AS1-del stable overexpression and HeLa cells with SPINT1-AS1 stable silencing were used to conduct RIP assays using a DROSHA antibody (5 μg per reaction; #3410, Cell Signaling Technology, Danvers, MA, United States), a DGCR8 antibody (5 μg per reaction; ab191875, Abcam, Hong Kong, China), and the Magna RIP RNA-Binding Protein Immunoprecipitation Kit.
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