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Dulbecco s mem nutrient mix f 12

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Dulbecco's MEM/Nutrient Mix F-12 is a basal medium that supports the growth and maintenance of various cell types. It is a combination of Dulbecco's Modified Eagle's Medium (DMEM) and Nutrient Mixture F-12 (Ham's F-12), providing a balanced formulation of nutrients, vitamins, and salts to sustain cell culture.

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Lab products found in correlation

Penicillin streptomycin, by Merck Group (1 mentions)

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Dulbecco’s MEM (36 citations) S-MEM (19 citations) F-12 nutrients (2 citations)

3 protocols using dulbecco s mem nutrient mix f 12

1

Culturing Human Neuroblastoma Cells

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BE (2)-M17 human neuroblastoma cells were routinely cultured in Dulbecco's MEM/Nutrient Mix F-12 (1:1) (Gibco BRL, Rockville, MD) supplemented with 15% fetal bovine serum and 1% penicillin-streptomycin (100 U/ml penicillin, 100 mg/ml streptomycin). The cells were maintained at 37°C in a humidified incubator with 5% CO2/95% air.
Ardah M.T., Paleologou K.E., Lv G., Abul Khair S.B., Kazim A.S., Minhas S.T., Al-Tel T.H., Al-Hayani A.A., Haque M.E., Eliezer D, & El-Agnaf O.M. (2014). Structure activity relationship of phenolic acid inhibitors of α-synuclein fibril formation and toxicity. Frontiers in Aging Neuroscience, 6, 197.
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2

Mammalian Cell Culture Conditions

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These cells were grown in Dulbecco’s MEM/Nutrient Mix F-12 (1:1) (Gibco BRL, Rockville, MD) supplemented by 15% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich). They were incubated at 37 °C in a 5% CO2/95% air humidified incubator to maintain their growth.
Ardah M.T., Ghanem S.S., Abdulla S.A., Lv G., Emara M.M., Paleologou K.E., Vaikath N.N., Lu J.H., Li M., Vekrellis K., Eliezer D, & El-Agnaf O.M. (2020). Inhibition of alpha-synuclein seeded fibril formation and toxicity by herbal medicinal extracts. BMC Complementary Medicine and Therapies, 20, 73.
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3

Culturing BE(2)-M17 Neuroblastoma Cells

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BE(2)-M17 human neuroblastoma cells were routinely cultured in Dulbecco's MEM/Nutrient Mix F-12 (1:1) (Gibco BRL, Rockville, MD) containing 15% fetal bovine serum and 1% penicillin-streptomycin (P/S; 100 U/ml penicillin, 100 mg/ml streptomycin). The cells were maintained at 37°C in a humidified incubator with 5% CO2/95% air.
Ardah M.T., Paleologou K.E., Lv G., Menon S.A., Khair S.B., Lu J.H., Safieh-Garabedian B., Al-Hayani A.A., Eliezer D., Li M, & El-Agnaf O.M. (2014). GINSENOSIDE RB1 INHIBITS FIBRILLATION AND TOXICITY OF ALPHA-SYNUCLEIN AND DISAGGREGATES PREFORMED FIBRILS. Neurobiology of disease, 74, 89-101.
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