Apyrase type 5

Collagen was purchased from Chrono-log Corporation (Havertown, PA, USA). The 2-MeSADP, thrombin, daphnetin, apyrase (type V), prostaglandin E₁ (PGE₁), sodium citrate, and acetylsalicylic acid (ASA) were bought from Sigma (St. Louis, MO, USA). Anti-phospho-cPLA₂ (Ser505), anti-cPLA₂, anti-phospho-ERK (Thr202/Tyr204), anti-ERK, Anti-phospho-AKT (Ser473), and anti-AKT antibodies were from Cell Signaling Technology (Beverly, MA, USA). Horseradish peroxidase-labeled secondary antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). TxB₂ ELISA kit was from Enzo Life Sciences (Exeter, UK). All additional chemicals were of reagent grade.

2-MeSADP, U46619, ADP, thrombin, serotonin, epinephrine, apyrase (type V), prostaglandin E₁ (PGE₁), sodium citrate, and ASA were bought from Sigma (St. Louis, MO, USA). Dr. Richard Farndale of the University of Cambridge provided CRP. AYPGKF was from Invitrogen (Carlsbad, CA, USA). Anti-phospho-Akt (Ser⁴⁷³), anti-phospho-ERK (Thr202/Tyr204), anti-Akt, anti-ERK, and anti-β-actin antibodies were from Cell Signaling Technology (Beverly, MA, USA). Horseradish peroxidase-labeled secondary antibody was bought from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All additional chemicals were of reagent grade.

All reagents were purchased from Thermo Fisher Scientific unless otherwise stated. 2-MeSADP, ADP, and Apyrase (type V) were purchased from Sigma (St. Louis, MO, USA). AR-C69931MX was a gift from the Medicines Company (Parsippany, NJ, USA). AYPGKF was purchased from GenScript (Piscataway, NJ, USA). Phosphorylated Akt S473 and phosphorylated VASP S157 were purchased from Cell Signaling Technologies (Beverly, MA, USA), while the total Akt antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The total VASP antibody was purchased from Origene (Rockville, MD, USA). Odyssey blocking buffer and secondary antibodies IRDye 800CW goat anti-rabbit and IRDye 680LT goat anti-mouse were purchased from Li-Cor (Lincoln, NE, USA). Collagen and Chronolume, used for the detection of secreted ATP, were purchased from Chrono-Log Corporation (Havertown, PA, USA). GTP-bound Rap1b was assessed using a kit from Cell Signaling and cAMP production was measured using a kit from Enzo. Prostaglandin E1 was also purchased from Enzo (New York, NY, USA). AseI was purchased from New England BioLabs (Ipswitch, MA, USA).