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2 protocols using germacrone

1

Evaluating Compounds in Hematology

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Normal and abnormal ZTOI were provided by the manufacturer. The standard compounds of β‐elemene, germacrone, curdione and furanodiene were purchased from Shanghai Yuanye Bio‐Technology Co., Ltd. Human Hb and dimethyl sulphoxide (DMSO) were purchased from Sigma‐Aldrich (St. Louis, MO, USA).
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2

Germacrone-Induced Autophagy Signaling

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Germacrone (purity >98%) was bought from Shanghai YuanYe Biotechnology Co. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) was bought from Bio Basic. Antibody against GAPDH was bought from Merck Millipore (Darmstadt, Germany). Antibodies against LC3B, mTOR, p-mTOR, Akt, and p-Akt were bought from Cell Signaling Technology (Danvers, MA). HRP-conjugated anti-rabbit antibody was bought from Promega (Madison, USA). An enhanced chemiluminescence (ECL) kit was bought from Biological Industries (Kibbutz Beit Haemek, Israel). Chloroquine and 3-methyladenine were purchased from Sigma Aldrich (St. Louis, MO). Goat anti-rabbit IgG H&L was bought from Abcam (Cambridge, United Kingdom).
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