Cells were lysed in Trizol (15596018; Thermo Fisher Scientific, Waltham, MA, USA) and RNA was isolated using Direct-zol RNA Miniprep kit (R2072; Zymo Research, Irvine, CA, USA) in accordance with manufacturers instructions. cDNA was synthesized using a High Capacity reverse transcription cDNA kit (4368813; Thermo Fisher Scientific). Quantitive real-time reverse transcription PCR was performed on an Applied Biosystems Prism 7900 using TaqMan and SYBR Green gene expression assays (Thermo Fisher Scientific)
Taqman and sybr green gene expression assays
Manufactured by Thermo Fisher Scientific
TaqMan and SYBR Green gene expression assays are laboratory tools used for the quantification of gene expression. TaqMan assays utilize probe-based detection, while SYBR Green assays rely on dye-based detection. Both methods provide sensitive and reliable quantification of target gene expression levels.
Automatically generated - may contain errors
2 protocols using taqman and sybr green gene expression assays
1
RNA Isolation and qRT-PCR Analysis
2
RNA Isolation and qRT-PCR Analysis
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!