α rhob

Manufactured by Santa Cruz Biotechnology

α-RhoB is a primary antibody that recognizes the RhoB protein. RhoB is a small GTPase that belongs to the Rho family of proteins. The α-RhoB antibody can be used to detect and study the expression and localization of RhoB in various experimental systems.

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Lab products found in correlation

α gapdh, by Cell Signaling Technology (2 mentions) α ve cadherin xp, by Cell Signaling Technology (2 mentions) α p44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) α rhoa, by Cell Signaling Technology (2 mentions) α rhoc, by Cell Signaling Technology (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Alexa fluor 647 secondary antibody, by Thermo Fisher Scientific (1 mentions) α vinculin, by Merck Group (1 mentions) Mouse α ubiquitin fk 2, by R&D Systems (1 mentions) Alexa fluor 488 secondary antibody, by Thermo Fisher Scientific (1 mentions) Hrp conjugated goat anti rabbit antibody, by Agilent Technologies (1 mentions) Alexa 488 secondary antibody, by Thermo Fisher Scientific (1 mentions) Mln4924, by Active Motif (1 mentions) Bay 11 7085, by Cayman Chemical (1 mentions) Acti stain 670 phalloidin, by Cytoskeleton (1 mentions) Csn5i 3, by Novartis (1 mentions) α p p65, by Cell Signaling Technology (1 mentions) α iκbα, by Cell Signaling Technology (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Y 27632, by Merck Group (1 mentions)

Spelling variants of this product

Rabbit α-RhoB antibody (2 citations)

2 protocols using α rhob

Immunostaining and Protein Analysis of RhoGTPases

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The following antibodies were used for immunostaining: α-VE-cadherin XP (#2500; Cell Signaling Technologies, Danvers, MA) and a-RhoB (#sc-8048; Santa Cruz Biotechnology, Santa Cruz, CA). Alexa Fluor 488 secondary antibody (anti-rabbit and anti-mouse) and Alexa Fluor 647 secondary antibody (anti-rabbit) (all Invitrogen) were the secondary antibodies.
The following antibodies were used for protein analysis: ß-actin (Merck), LDLR (Biovision), and SQLE (Proteintech). α-p44/42 MAPK (ERK1/2) (#9102), α-RhoA (#2117), α-RhoC (#3430), and α-GAPDH (#2118; Cell Signaling Technologies), α-RhoB (#sc-180, #sc-8048; Santa Cruz Biotechnology), α-HA (H3663), and α-vinculin (V4139; Sigma-Aldrich), α-Fbxw7 (ab171961; Abcam) and mouse α-ubiquitin (FK-2; Boston Biochem). HRP-conjugated goat–anti-rabbit antibody and goat–anti-mouse (Dako) were used as secondary antibodies.
For inhibition of geranylgeranylation and farnesylation, GGTI-298 and FTI-277 HCL (both Selleck Chemicals) were used. For normalization of prenylation in the FBXW7 knockdown cells, GGPP or FPP were applied (Sigma).
The siRNAs used were ON-TARGET plus Nontargeting pool (siNT), ON-TARGET plus Human FBXW7 pool (siFBXW7), ON-TARGET plus Human RHOA siRNA pool (siRhoA), ON-TARGET plus Human RHOB siRNA pool (siRhoB), and ON-TARGET plus Human FBXW7 siRNA—Set of 4 Upgrade (all Dharmacon/GE-Healthcare, Lafayette, CO).

Pronk M.C., Majolée J., Loregger A., van Bezu J.S., Zelcer N., Hordijk P.L, & Kovačević I. (2019). FBXW7 regulates endothelial barrier function by suppression of the cholesterol synthesis pathway and prenylation of RhoB. Molecular Biology of the Cell, 30(5), 607-621.

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Investigating Cell-Cell Adhesion Regulators

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The following antibodies were used in this study: αVE-cadherin XP (#2500), αRhoA (#2117), αRhoC (#3430), αCullin-3 (#2759), αp44/42 MAPK (ERK1/2) (#9102), αpMLC2 Ser19 (#3671), αGAPDH (#2118), αIκBα (#4841) and αpp65 (#93H1) (all from Cell Signaling Technology); αRhoB (#sc-8048 and #sc-180), αCullin-1 (#sc-17775), αICAM (#sc-8439) (all from Santa Cruz Biotechnology); αCullin-2 (#610778) and αp38 (#61268) (BD Transduction Laboratories) and αpp38 (#09–272) (Millipore).
Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse antibodies (Dako) were used as secondary antibodies for western blotting. For immunofluorescent staining DAPI (Thermo Fisher scientific), Alexa 488-secondary antibody (anti-rabbit and anti-mouse, Invitrogen) and Acti-stain 670 phalloidin (Cytoskeleton) were used.
The following inhibitors and cytokines were used in this study: CSN5i-3 (Novartis), MLN4924 (Active Biochem), Y27632 (#Y0503) (Sigma), TNF-α (#300-01 A) (PeproTech), BAY11-7085 (Cayman Chemical).

Majolée J., Pronk M.C., Jim K.K., van Bezu J.S., van der Sar A.M., Hordijk P.L, & Kovačević I. (2019). CSN5 inhibition triggers inflammatory signaling and Rho/ROCK-dependent loss of endothelial integrity. Scientific Reports, 9, 8131.

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