Adhesion microscope slides

Manufactured by Thermo Fisher Scientific

Sourced in Germany

Adhesion microscope slides are a type of laboratory equipment designed for the analysis and observation of cell adhesion, cell-cell interactions, and cell-substrate interactions using microscopy techniques. These slides provide a uniform and optimized surface for cells to adhere to, enabling researchers to study the dynamics and mechanisms of cell adhesion in a controlled environment.

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Lab products found in correlation

Cm1860 cryostat, by Leica (1 mentions) Blood glucose meter, by Roche (1 mentions) Au480 automatic chemistry analyzer, by Beckman Coulter (1 mentions) Novolink polymer detection system l, by Leica (1 mentions) Calbindin, by Cell Signaling Technology (1 mentions)

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Microscope slides (156 citations) Superfrost Plus Adhesion Microscope Slides (15 citations) Polysine microscope adhesion slides (5 citations) Adhesion slides (4 citations)

2 protocols using adhesion microscope slides

Kidney tissue analysis protocol

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The FBG was determined using a blood glucose meter (Roche, Basel, Switzerland). Glycosylated hemoglobin (HbA1c) was detected with a Quo-Test HbA1c Analyzer (QUOTIENT Diagnostics Ltd. Walton-on-Thames, Surrey, UK). The urine creatinine and urea nitrogen were measured using an AU480 automatic chemistry analyzer (Beckman Coulter Inc., Brea, CA, USA).
The frozen kidney tissues were cut into 8 μm serial sections at −22 °C using a Leica CM1860 cryostat (Leica Microsystems Ltd., Wetzlar, Germany) and mounted onto adhesion microscope slides (Thermo Scientific, CA, USA). Hematoxylin and eosin (H&E) staining of kidney sections was performed to reveal the histopathological lesions.

Zhang X., Liu Y., Yang S., Gao X., Wang S., Wang Z., Zhang C., Zhou Z., Chen Y., Wang Z, & Abliz Z. (2023). Comparison of Local Metabolic Changes in Diabetic Rodent Kidneys Using Mass Spectrometry Imaging. Metabolites, 13(3), 324.

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Immunohistochemistry of Cerebellar Tissues in SCA 17 Mice

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Wild-type and SCA 17 transgenic mice were cardiac perfused first with PBS and then fixed with 4% formaldehyde (EM grade) at the age of 24 weeks. Cerebellar specimens were then embedded in paraffin, cut into sections (5 μm), and placed on adhesion microscope slides (Thermo Fisher Scientific). By using the heat-induced epitope retrieval method, the sections were separately stained at room temperature for 1 hour with antibodies of calbindin (1:500; Cell Signaling Technology) or TBP (1:1,000; Santa Cruz Biotechnology Inc.). Detection was performed by incubation with biotinylated secondary antibodies (Novolink™ Polymer Detection System l; Leica Microsystems, Wetzlar, Germany), for 30 minutes at room temperature, followed by 30-minute incubation with avidin–biotin–HRP complex (Novolink™ Polymer Detection System l). Visualization was performed with 3,3′-diaminobenzidine Chromogen (Novolink™ Polymer Detection System l) and counterstained with hematoxylin (Novolink™ Polymer Detection System l) following the supplier’s protocol.

Huang D.S., Lin H.Y., Lee-Chen G.J., Hsieh-Li H.M., Wu C.H, & Lin J.Y. (2016). Treatment with a Ginkgo biloba extract, EGb 761, inhibits excitotoxicity in an animal model of spinocerebellar ataxia type 17. Drug Design, Development and Therapy, 10, 723-731.

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