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Human cxcl5 ena 78 quantikine elisa kit

Manufactured by R&D Systems
Sourced in United States

The Human CXCL5/ENA-78 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of human CXCL5/ENA-78 levels in cell culture supernates, serum, and plasma.

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2 protocols using human cxcl5 ena 78 quantikine elisa kit

1

CXCL5/ENA-78 Quantification in LPS-treated LECs

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LECs were plated in 12 well plates and treated with LPS (100 ng/mL) for 24 h and LEC-CM and LPS-LEC-CM was collected as described above. ELISA was carried out using the Human CXCL5/ENA-78 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), according to manufacturer’s instructions. Values were represented as pg/mL.
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2

Quantification of Cytokines in Cell Supernatant

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Quantitation of IL30, CXCL5 and IGF1, in the supernatant derived from murine or human PC cells, was carried out using the following ELISA kits, according to manufacturer’s protocols: Human CXCL5/ENA-78 Quantikine ELISA Kit (#DX000, R&D Systems, Minneapolis, MN, USA); human IGF1 ELISA Kit (#ab211651, Abcam, Cambridge, UK); and mouse Interleukin-27 subunit alpha ELISA Kit (#CSB-E08466m, Cusabio, Wuhan, China).
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