Gibco rpmi medium

Human pre-BCR⁺/E2A-PBX1⁺ ALL cell lines RCH-ACV (RRID: CVCL_1851) and 697 (RRID: CVCL_0079) and pre-BCR⁻/E2A-PBX1⁻ ALL cell lines REH (RRID: CVCL_1650), HAL-01 (RRID: CVCL_1242) and SEM (RRID: CVCL_0095) were obtained from DSMZ (Leibniz Institute, Braunschweig, Germany) and cultured in Gibco™ RPMI medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, 100 µg/mL streptomycin and 2 mM L-Glutamine. The SEM cell line was cultured in Gibco™ IMDM medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, 100 µg/mL streptomycin, 4 mM L-Glutamine and 25 mM HEPES. Dasatinib-resistant cell lines were generated and described previously [12 (link)]. Two independent dasatinib-resistant clones were generated in parallel after the incubation of the pre-BCR⁺/E2A-PBX1⁺ RCH-ACV cell line with increasing concentrations of dasatinib for at least 9 months. Global transcriptomic profiles were generated to study differences between both clones in previous studies [12 (link)]. All human cell lines have been authenticated using STR (or SNP) profiling within the last three years. All experiments were performed with mycoplasma-free cells.

The pheochromocytoma cell line (PC 12), derived from rat adrenal medulla, was purchased from the American Type Culture Collection (ATCC, Manassas, Virginia, United States of America). PC 12 cells were cultured in complete Gibco™ RPMI medium (Thermo Fisher Scientific, Scoresby, Victoria, Australia) supplemented with 10% Gibco™ horse serum (HS; Thermo Fisher Scientific), 5% Gibco™ foetal bovine serum (FBS; Thermo Fisher Scientific) and 1% Gibco™ penicillin/streptomycin (PS; Thermo Fisher Scientific). Supplements were stored as aliquots at −20 °C. Stock solutions of PC 12 cells were prepared in a medium containing 90% FBS and 10 % DMSO and stored in liquid nitrogen. The cells were maintained at a temperature of 37 °C under a 5% CO₂ atmosphere in a 95% humidified incubator. The medium was changed every two days and passaged accordingly when the confluence reached 90%.

Pheochromocytoma cells are derived from the rat adrenal medulla (Tharushi Perera et al., 2022 ▸ ). The PC 12 cell line used in this study was purchased from the American Type Culture Collection (ATCC, USA) and cultured in a complete Gibco RPMI medium (Thermo Fisher Scientific, Australia) supplemented with 10% Gibco horse serum (Thermo Fisher Scientific, Australia, HS), 5% Gibco foetal bovine serum (Thermo Fisher Scientific, Australia, FBS) and 1% Gibco penicillin/streptomycin (Thermo Fisher Scientific, Australia). Supplements were stored as aliquots at −20°C. Stock solutions of the PC 12 cells were prepared in a medium containing 90% FBS and 10% DMSO and stored in liquid nitro­gen. The cells were maintained at 37°C with 5% CO₂ in a 95% humidified incubator. The medium was changed every two days and passaged accordingly when the confluence reached 90%.