α tubulin dm1a

Manufactured by Cell Signaling Technology

Sourced in United States

α-tubulin (DM1A) is a monoclonal antibody that recognizes the α-tubulin protein, a component of the microtubule cytoskeleton. This antibody is commonly used in research applications to detect and visualize α-tubulin in various cell types and tissues.

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Lab products found in correlation

Horseradish peroxidase conjugated anti mouse and anti rabbit igg, by Cell Signaling Technology (2 mentions) Molecular imaging software, by Carestream (2 mentions) Ahr d5s6h, by Cell Signaling Technology (1 mentions) β actin, by Beyotime (1 mentions) Ch 223191, by Selleck Chemicals (1 mentions) Bay 11 7085, by MedChemExpress (1 mentions) Rela d14e12, by Cell Signaling Technology (1 mentions) Iκbα l35a5, by Cell Signaling Technology (1 mentions) Phospho iκbα 14d4, by Cell Signaling Technology (1 mentions) Laminb1 d9v6h, by Cell Signaling Technology (1 mentions) Gapdh, by Beyotime (1 mentions) Odyssey infrared scanner, by LI COR (1 mentions) Irdye 680rd goat anti mouse, by LI COR (1 mentions) Odyssey version 3, by LI COR (1 mentions) Tyrosine hydroxylase, by Abcam (1 mentions) Irdye 800cw goat anti rabbit, by LI COR (1 mentions) P erk thr202 tyr204, by Cell Signaling Technology (1 mentions) Erk1 2 c 9, by Santa Cruz Biotechnology (1 mentions) Connexin 43, by Santa Cruz Biotechnology (1 mentions) Max h2 sc 8011, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

α-tubulin (597 citations) α-tubulin (clone DM1A, (4 citations) α-tubulin (DM1A) mouse mAb (2 citations) Mouse anti-human α-tubulin (clone DM1A) monoclonal antibody (2 citations)

9 protocols using α tubulin dm1a

Investigating HTLV-1 Activation Pathway

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CH-223191 was purchased from Selleck. L-kynurenine and BAY11-7085 were purchased from MedChemExpress. Antibodies were used as following: AHR (D5S6H; Cell Signaling Technology), RelA (D14E12; Cell Signaling Technology), IκBα (L35A5; Cell Signaling Technology), phospho-IκBα (14D4; Cell Signaling Technology), α-Tubulin (DM1A; Cell Signaling Technology), LaminB1 (D9V6H; Cell Signaling Technology), HTLV-1 Tax (1A3; Abcam), HTLV-1 gp46 (67/5.5.13.1; Abcam), HTLV-1 p24 (46/3.24.4; Abcam), HTLV-1 p19 (TP-7; Abcam), β-Actin (AF0003; Beyotime Biotechnology), GAPDH (AF0006; Beyotime Biotechnology).

Hong W., Cheng W., Zheng T., Jiang N, & Xu R. (2020). AHR is a tunable knob that controls HTLV-1 latency-reactivation switching. PLoS Pathogens, 16(7), e1008664.

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Quantitative Protein Profiling in Cells

Cited 2 times

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Protein extractions were performed as previously described²⁸ (link)^,²⁹ (link). Primary antibodies p-ERK (Thr202/Tyr204, Cell Signaling, #4370S; 1:1000 dilution), ERK1/2 (C-9, Santa Cruz Biotechnology, SC-514302; 1:200 dilution), tyrosine hydroxylase (Abcam, ab75875; 1:1000 dilution), adiponectin (rabbit polyclonal, home-made), Connexin43 (Santa Cruz Biotechnology, SC-6560-R) and α-tubulin (DM1A, Cell Signaling, #3873S) were used. Protein abundance was detected using one of the following secondary antibodies: goat anti-mouse IRDye 680RD (LI-COR Biosciences, 926-68070), goat anti-rabbit IRDye 800CW (LI-COR Biosciences, 925-32211) at 1:10,000 dilutions. Antibody decorated membranes were then visualized on a LI-COR Odyssey infrared scanner (LI-COR Biosciences). The scanned data were analyzed using Odyssey Version 3.0 software (LI-COR Biosciences).

Zhu Y., Li N., Huang M., Chen X., An Y.A., Li J., Zhao S., Funcke J.B., Cao J., He Z., Zhu Q., Zhang Z., Wang Z.V., Xu L., Williams K.W., Li C., Grove K, & Scherer P.E. (2022). Activating Connexin43 gap junctions primes adipose tissue for therapeutic intervention. Acta Pharmaceutica Sinica. B, 12(7), 3063-3072.

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Comprehensive Antibody Panel for Western Blotting

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Antibodies used for Western blotting: YAP [D8H1X (#14074) or 1A12 (#12395)], TAZ [D3I6D (#70148) or E5P2N (#71192)], Myc-tag [9B11 (#2276) or 71D10 (#2278)], panTEAD [D3F7L (#13295)], Rap1-interacting factor 1 (RIF1) [D2F2M (#95558)], GAPDH [D16H11 (#5174)], α-Tubulin [DM1A (#3873)], pS139 H2A.X [20E3 (#9718)], Ku70 [D10A7 (#4588)], and Ku80 (#2753) from Cell Signaling Technology. MAX [(H2) sc-8011] from Santa Cruz Biotechnology. Secondary antibodies: Goat anti-mouse (926–3220, Li-cor) and Goat-anti-rabbit (926–68071, Li-Cor).
Antibody used for immunoprecipitation: Myc-tag [9B11 (#2276) or 71D10 (#2278), Cell Signaling Technology], Mouse (G3A1) mAb IgG1 Isotype Control (#5415, Cell Signaling Technology).
Antibodies used for immunofluorescence: panTEAD [D3F7L (#13295), Cell Signaling Technologies], RIF1 (NBP2-26219, Novus biologicals), pS139 H2A.X (05–636, Millipore-Sigma).

Calses P.C., Pham V.C., Guarnaccia A.D., Choi M., Verschueren E., Bakker S.T., Pham T.H., Hinkle T., Liu C., Chang M.T., Kljavin N., Bakalarski C., Haley B., Zou J., Yan C., Song X., Lin X., Rowntree R., Ashworth A., Dey A, & Lill J.R. (2023). TEAD Proteins Associate With DNA Repair Proteins to Facilitate Cellular Recovery From DNA Damage. Molecular & Cellular Proteomics : MCP, 22(2), 100496.

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Antibodies and siRNA Sequences for Ezrin Study

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Antibodies against ezrin, ezrin pT567, acetylated-Lysine, and α-tubulin (DM1A) were purchased from Cell Signaling Technology. Anti-Na,K-ATPase and anti-GFP antibodies were purchased from Santa Cruz Biotechnology. Anti-FLAG-tag (M2) antibody was from Sigma. Phalloidin was purchased from Invitrogen, and ezrin pS66 antibody was described before (Yu et al., 2014 (link); Fang et al., 2017). The PCAF siRNA and ezrin siRNA sequence were described before (Ding et al., 2010 (link); Xia et al., 2012 (link)).

Song X., Wang W., Wang H., Yuan X., Yang F., Zhao L., Mullen M., Du S., Zohbi N., Muthusamy S., Cao Y., Jiang J., Xia P., He P., Ding M., Emmett N., Ma M., Wu Q., Green H.N., Ding X., Wang D., Wang F, & Liu X. (2019). Acetylation of ezrin regulates membrane–cytoskeleton interaction underlying CCL18-elicited cell migration. Journal of Molecular Cell Biology, 12(6), 424-437.

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Investigating Protein Posttranslational Modifications

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CPT, CBP, Etop, Dox, and gliotoxin were purchased from Sigma-Aldrich. Human and mouse recombinant TNFα was from CalBiochem and prepared in phosphate-buffered saline containing 0.1% bovine serum albumin fraction V (Sigma-Aldrich) to a final stock concentration of 10 μg/mL IgGs against c-Myc (9E10), GFP (B2), IκB-α (C-21), GST (B-14), p65 (F-6), ubiquitin (P4D1), and CYLD (H6) purchased from Santa Cruz Biotechnology. IgGs against HA (C29F4), His (D3I10), p-p65 (Ser536) (93H1), p-IκBα (Ser32) (14D4), K63-Ub (D7A11), K48-Ub (D9D5), cleaved caspase-3 (Asp175) (5A1E), RNF31 (E6M5B), OTULIN (14127s), and α-tubulin (DM1A) were purchased from Cell Signaling Technology. Anti-HOIL-1 antibody (2E2) and anti-linear ubiquitin antibody (LUB9) were obtained from Millipore Sigma. Anti-SHARPIN antibody (ab197853) was purchased from ABCAM. N-ethylmaleimide (catalog no. 23030) and IAM (catalog no. A39271) were purchased from Thermo Scientific.

Li M., Li L., Asemota S., Kakhniashvili D., Narayanan R., Wang X, & Liao F.F. (2022). Reciprocal interplay between OTULIN–LUBAC determines genotoxic and inflammatory NF-κB signal responses. Proceedings of the National Academy of Sciences of the United States of America, 119(33), e2123097119.

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Antibody Panel for Autophagy Signaling

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The following antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA): LC3A/B (D3U4C) (#12741), total 4E-BP1 (53H11) (#9644), phospho-4E-BP1 (Thr37/46) (#2855), total p70-S6K (49D7) (#2708), phospho-p70-S6K (Thr389) (#9205), total mTOR (#2983), phospho-mTOR (Ser2448) (#1356), Alix (3A9) (#2171), Flotillin-1 (D2V7J) (#18,634), β-actin (13E5) (#4970) for immunofluorescence, α-tubulin (DM1A) (#3873) for immunofluorescence. Other antibodies used: LAMP1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-19992), M6PR (Abcam, Cambridge, UK, ab32815) for immunofluorescence, M6PR (BBI Life Sciences, Shanghai, China, D154247) for western blotting, CD63 (BioLegend, San Diego, CA, USA, 143,902), Cathepsin D (Abcam, ab75852), Vinculin (PTM Biolabs, Hangzhou, China, PTM-5168), β-Actin (Ruiying Biological, Suzhou, China, RLT0099) for immunoblotting.

Sun S., Li Q., Liu G., Huang X., Li A., Guo H., Qi L., Zhang J., Song J., Su X, & Zhang Y. (2024). Endosomal protein DENND10/FAM45A integrates extracellular vesicle release with cancer cell migration. BMC biology, 22(1).

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Muscle Metabolism Protein Analysis

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The following primary antibodies were used: PHD-2/Egln1 antibody (3293, Cell Signaling Technology), EGLN3/PHD3 antibody (NB100-303, Novus), Monoclonal Anti-Myosin slow (M8421, Sigma), MYH2 antibody (A4.74, Santa Cruz Biotechnology), Anti-PGC-1α (AB3243, Millipore), Anti-Calcineurin pan A (07-1491, Millipore), HIF-1α (NB100-479, Novus), NFATc1 (sc-7294, Santa Cruz Biotechnology), MEF2 (sc-313, Santa Cruz Biotechnology), myoglobin (FL-154, Santa Cruz Biotechnology), β-actin (AC-74, Sigma-Aldrich), GAPDH (14C10, Cell Signaling Technology), and α-tubulin (DM1A, Cell Signaling Technology).

Shin J., Nunomiya A., Kitajima Y., Dan T., Miyata T, & Nagatomi R. (2016). Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway. Skeletal Muscle, 6, 5.

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Western Blot Analysis of Mitochondrial Proteins

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Western blotting was performed as previously described^{17 (link)} using rabbit primary antibodies to SIRT3 (#5490, Cell Signaling Technology, Danvers, MA, USA), OPA1 (#80471, Cell Signaling Technology), cleaved caspase-3 (Asp175) (Cell Signaling Technology) and PINK1 (Sigma-Aldrich) diluted 1:1000. Mouse monoclonal primary antibodies to α-tubulin (DM1A) (Cell Signaling Technology) and β-actin (A5441, clone AC-15) (Sigma-Aldrich) were diluted, respectively, 1:1000 and 1:5000 before use. Horseradish peroxidase conjugated anti-mouse and anti-rabbit IgG (Cell Signaling Technology) secondary antibodies were used at 1:2,000 dilution. A semi quantitative measurement of band intensity was performed using the Carestream Molecular Imaging Software (Carestream Molecular Imaging, New Haven, CT, USA) and Fiji^{36 (link)}.

Mwangi S.M., Li G., Balasubramaniam A., Merlin D., Dawson P.A., Jang Y.C., Hart C.M., Czaja M.J, & Srinivasan S. (2022). Glial cell derived neurotrophic factor prevents western diet and palmitate-induced hepatocyte oxidative damage and death through SIRT3. Scientific Reports, 12, 15838.

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Quantitative Western Blot Analysis

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Western blotting was performed as previously described (11 (link)) using rabbit primary antibodies to Atg5 (D5F5U), Atg7, Beclin-1, cleaved caspase-3 (Asp175) LC3A/B (D3U4C), total and phospho-mTOR (Ser 2448), total and phospho-p70SK6 (Thr389), total and phospho-4E-BP1 (Ser65) (Cell Signaling Technologies), and p62/SQSTM1 (Sigma-Aldrich) diluted 1:1000. Mouse monoclonal primary antibodies to α-tubulin (DM1A) (Cell Signaling Technologies, Danvers, MA) and β-actin (A5441, clone AC-15) (Sigma-Aldrich) were diluted, respectively, 1:1000 and 1:5000 before use. Horseradish peroxidase conjugated anti-mouse and anti-rabbit IgG (Cell Signaling Technologies) secondary antibodies were used at 1:2,000 dilution. A semi quantitative measurement of band intensity was performed using the Carestream Molecular Imaging Software (Carestream Molecular Imaging, New Haven, CT).

Mwangi S.M., Li G., Ye L., Liu Y., Reichardt F., Yeligar S.M., Hart C.M., Czaja M.J, & Srinivasan S. (2019). Glial Cell Line Derived Neurotrophic Factor Enhances Autophagic Flux in Mouse and Rat Hepatocytes and Protects Against Palmitate Lipotoxicity. Hepatology (Baltimore, Md.), 69(6), 2455-2470.

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