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Sc3052

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

SC3052 is a laboratory instrument designed for analytical applications. The device is capable of performing precise measurements and data analysis. No further details on the intended use or capabilities of the product are provided.

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Lab products found in correlation

Cholesterol, by Avanti Polar Lipids (2 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Alexa 633, by Thermo Fisher Scientific (1 mentions) Anti pdi, by Merck Group (1 mentions) Alexa 555, by Thermo Fisher Scientific (1 mentions) Anti giantin, by Fortrea (1 mentions) Melatonin m5250, by Merck Group (1 mentions) Cd34 sc 74499, by Santa Cruz Biotechnology (1 mentions) β actin a5441, by Merck Group (1 mentions)

4 protocols using sc3052

1

Immunofluorescence Staining of Organelles

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The following antibodies were used: mouse monoclonal anti-GM130 (Transduction Laboratories; 1:500); rabbit polyclonal anti-GM130 (23 (link)) (from A. De Matteis, Telethon Institute of Genetics and Medicine; 1:1000); rabbit polyclonal anti-giantin (Covance; 1:500) and rabbit polyclonal anti-PDI (Sigma Aldrich; 1:500); mouse monoclonal anti-VSV-G (clone P5D4, Sigma Aldrich; 1:100); rabbit polyclonal anti-VSV-G luminal domain (23 (link)) (from A. De Matteis; 1:100); mouse monoclonal anti-PC-I (Developmental Studies Hybridoma Bank, Iowa University; 1:100); mouse monoclonal anti-PrP antibodies 6H4 (Thermo Fisher Scientific; 1:500) and 12B2 (Central Veterinary Institute; 1:400 for immunofluorescence and 1:5000 for western blot). Secondary antibodies were conjugated with Alexa-488, Alexa-555, or Alexa-633 fluorophores (Invitrogen). The Src family activator (sc-3052) was from Santa Cruz Biotechnology.
Restelli E., Capone V., Pozzoli M., Ortolan D., Quaglio E., Corbelli A., Fiordaliso F., Beznoussenko G.V., Artuso V., Roiter I., Sallese M, & Chiesa R. (2021). Activation of Src family kinase ameliorates secretory trafficking in mutant prion protein cells. The Journal of Biological Chemistry, 296, 100490.
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2

In Vitro Reconstitution of Membrane Proteins

Cited 2 times
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The src family activator (Santa Cruz, #SC3052) has been described 19 (link). GDP and GTP were purchased (Sigma-Aldrich). DOPC (dioleoylphosphocholine), DOPE (dioleoylphosphatidylethanolamine), DOPS (dioleoylphosphatidylserine), PA (phosphatidic acid) and cholesterol were also purchased (Avanti Polar Lipid). Peptides encoding the Wbp1 tail (wild-type, KKLETFKKTN; mutant, KKLETFSSTN) were synthesized (GenScript). Culturing of HeLa cells have been described 9 (link),14 (link),15 (link).
Park S.Y., Yang J.S., Schmider A.B., Soberman R.J, & Hsu V.W. (2015). Coordinated regulation of bidirectional COPI transport at the Golgi by cdc42. Nature, 521(7553), 529-532.
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3

In Vitro Reconstitution of Membrane Proteins

Cited 2 times
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The src family activator (Santa Cruz, #SC3052) has been described 19 (link). GDP and GTP were purchased (Sigma-Aldrich). DOPC (dioleoylphosphocholine), DOPE (dioleoylphosphatidylethanolamine), DOPS (dioleoylphosphatidylserine), PA (phosphatidic acid) and cholesterol were also purchased (Avanti Polar Lipid). Peptides encoding the Wbp1 tail (wild-type, KKLETFKKTN; mutant, KKLETFSSTN) were synthesized (GenScript). Culturing of HeLa cells have been described 9 (link),14 (link),15 (link).
Park S.Y., Yang J.S., Schmider A.B., Soberman R.J, & Hsu V.W. (2015). Coordinated regulation of bidirectional COPI transport at the Golgi by cdc42. Nature, 521(7553), 529-532.
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4

Investigating Angiogenic Signaling Pathways

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Recombinant human VEGF (100-20) was bought from PeproTech (Rocky Hill, NJ, USA). VEGFR2 (07-158), phospho-VEGFR2 (orb106137), CD133 (orb13002), and β-actin (a5441) antibodies, and melatonin (M5250) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phospho-FAK (3283S) antibodies was purchased from Cell Signaling (Danvers, MA, USA). FAK (sc-1688), phospho-c-Src (sc-12928-R), c-Src (2105S), phospho-c-Jun (sc-822), c-Jun (sc-74543), phospho-p65 (sc-101752), p65 (sc-8008), CD31 (sc-18916), and CD34 (sc-74499) antibodies, as well as the angiotensin II (FAK activator; sc-363643) and c-Src activator (sc-3052), were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The NF-κB activator (prostratin; ab120880) was purchased from Abcam (Cambridge, MA, USA). PDGF-BB antibody (MBS9404630) was purchased from MyBioSource (San Diego, CA, USA). The VEGFR2 short hairpin RNA (shRNA) plasmid was purchased from the National RNAi Core Facility Platform (Taipei, Taiwan).
Lin L.W., Wang S.W., Huang W.C., Huynh T.K., Lai C.Y., Ko C.Y., Fong Y.C., Lee J.J., Yang S.F, & Tang C.H. (2023). Melatonin Inhibits VEGF-Induced Endothelial Progenitor Cell Angiogenesis in Neovascular Age-Related Macular Degeneration. Cells, 12(5), 799.
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