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Poly ethyleneimine branched pei

Manufactured by Merck Group
Sourced in United States

Poly(ethyleneimine) (branched PEI) is a versatile, water-soluble polymer that is commonly used in various laboratory applications. It features a highly branched structure with a high density of amine groups. This polymer can be utilized for a range of purposes, including but not limited to transfection, nanoparticle formation, and surface modification.

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3 protocols using poly ethyleneimine branched pei

1

Polymer Synthesis and Biomedical Applications

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4,4′-Azobis-(4-cyanovaleric acid) (V501), 4-cyano-4-(phenylcarbonothioylthio) pentanoic acid (RAFT agent), poly(ethyleneimine) (branched PEI, molecular weight: 25 kDa), NHSS, and EDC were purchased from Sigma-Aldrich Co., St Louis, MO, USA. Sylgard®184 from Dow Corning was used to prepare PDMS, which was cured for 12 hours using curing agent and elastomer base in the ratio of 10:1. Trypticase soy broth and trypticase soy agar were obtained from Hangzhou Baisi Corporation. A Millipore Milli-Q system (Millipore, Billerica, MA, USA) was used to produce ultrapure distilled water.
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2

Curcumin-Loaded Nanoparticle Synthesis

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Curcumin (≧ 94% purity), polyethylene glycol (PEG, MW 1500 and 8000), polyethyleneimine (branched PEI, MW 25,000) and 3,3′-dioctadecyloxacarbocyanine (DiO) were purchased from Sigma-Aldrich (St. Louis, MO). 1,2-Dioleoyl-sn-Glycero-3-Phosphocholine (DOPC) and 1,2-Dilauroyl-sn-Glycero-3-Phosphocholine (DLPC) were purchased from Avanti Polar Lipids (Alabaster, AL). Herceptin was purchased from Roche (Basel, Switzerland). Rabbit polyclonal antibody to human IgG F(c)-FITC was purchased from Acris Antibodies GmbH (Herford, Germany).
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3

Synthesis of Gold-Iron Oxide Nanoparticles

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Gold(III) chloride trihydrate
(HAuCl4·3H2O, 99.8% from Strem Chemicals
Inc.), iron(III) acetylacetonate (Fe(acac)3, 99% from Sigma-Aldrich),
oleic acid (OA, 90%, Aldrich), oleylamine (Ol-NH2, 98%, Aldrich),
polyethyleneimine branched (PEI, average MW ∼ 25,000 from Sigma-Aldrich),
benzyl ether (Bz2O, 98%, Aldrich), dimethyl sulfoxide anhydrous
(DMSO, ≥99.9%, Aldrich), ethanol (EtOH, 99.8%, Fluka), and
toluene (99%, Aldrich) were used without any further purification.
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