WST-1 colorimetric assay for cell proliferation (Roche Diagnostics GmbH, Mannheim, Germany, reagent kit cat. 11644807001) is based on the viable cells’ ability to cleave the bright red-colored stable tetrazolium salt WST-1 to dark red soluble formazan. This bioreduction occurs under the influence of mitochondrial dehydrogenases (depends mostly on production of NAD(P)H in viable cells). The amount of formazan dye formed correlates directly with the number of metabolically active cells in the culture and is measured by absorbance (λ = 450 nm) following 1 h incubation of cells with the reagent.
Wst 1 colorimetric assay for cell proliferation
Manufactured by Roche
Sourced in Germany
The WST-1 colorimetric assay is a laboratory tool used to measure cell proliferation. It provides a quantitative assessment of viable cells in a sample by detecting the conversion of a tetrazolium salt into a colored formazan product. The assay is based on the ability of viable cells to metabolize the WST-1 reagent, resulting in a measurable color change that can be detected spectrophotometrically.
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2 protocols using wst 1 colorimetric assay for cell proliferation
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Cell Proliferation Assay using WST-1
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Cell Proliferation Assay using WST-1
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WST-1 colorimetric assay for cell proliferation (Roche Diagnostics GmbH, Mannheim, Germany, reagent kit cat. 11644807001) is based on the viable cells’ ability to cleave the bright red-colored stable tetrazolium salt WST-1 to dark red soluble formazan. This bioreduction occurs under the influence of mitochondrial dehydrogenases (depends mostly on production of NAD(P)H in viable cells). The amount of formazan dye formed correlates directly with the number of metabolically active cells in the culture and is measured by absorbance (λ = 450 nm) following 1 h incubation of cells with the reagent.
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