Cd206 fitc

Macrophages from peritoneal exudate derived from mice in the TGC model (0.5 × 10⁶ cells) were resuspended in FACS buffer (PBS supplemented with 0.5% bovine serum albumin and 0.1% NaN₃) and were labeled with the antibody mixes from panel 1 (CD45-APC; F4/80-Viogreen; MHCII-Vioblue; LFA-1-PeVio770; CD64-PE; CD16/32-Viobright FITC) (Miltenyi Biotec, Bergisch Gladbach, Germany) and panel 2 (CD45-APC; F4/80-Viogreen; CD64-PE; MHCII-Vioblue; CD38-PeVio770; CD206-FITC) (Miltenyi Biotec, Bergisch Gladbach, Germany) for 20 min at 4°C. Cells were then washed with FACS buffer and the relative fluorescence intensities were determined using a MACSQuant Vyb cytometer equipped with FlowLogic software (Miltenyi Biotec, Bergisch Gladbach, Germany).

THP-1 cells were tested for cell surface antigen expression using flow cytometry. The cells were harvested and rinsed with PBS, after which CD206-FITC (Miltenyi Biotec, Bergisch Gladbach, Germany) and 7-AAD (eBioscience, Inc., San Diego, CA, USA) were added. Thereafter, the cells were incubated at 4 °C for 30 min in the dark. The stained cells were analyzed using CytoFLEX (Beckman Coulter Inc., Brea, CA, USA).