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Click it plus alexa flour 647 assay kit

Manufactured by Thermo Fisher Scientific
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The Click-iT Plus Alexa Fluor 647 Assay Kit is a labeling kit designed to detect and quantify cell proliferation. The kit enables the incorporation of a modified nucleoside, EdU (5-ethynyl-2'-deoxyuridine), into newly synthesized DNA during the S phase of the cell cycle. The incorporated EdU can then be detected using a copper-catalyzed click reaction with the Alexa Fluor 647 dye, providing a fluorescent signal that can be measured using flow cytometry or microscopy.

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Lab products found in correlation

Lsr fortessa 2, by BD (2 mentions)

Close spelling variants of this product

Click-iT Plus EdU Alexa Flour 647 Flow Cytometry Assay Kit Alexa Flour 647 Alexa Flours Alexa 647 PLUSTM

2 protocols using click it plus alexa flour 647 assay kit

1

Phenotypic Characterization of Antigen-Specific T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were first blocked for unspecific binding with αCD16/32 followed by staining with NP366–374/Db tetramer conjugated to allophycocyanin (APC) or Brilliant Violet 421 (BV421). Tetramer labelled cells was incubated with fluorophore conjugated antibodies CD8α (clone 53–6.7), CD8β (clone H35 17.2) CD44 (clone IM7), CD45.1 (clone 30-F11), CD45.2 (clone 104) CD69 (clone H1.2F3), CD103 (clone 2E7), PD-1 and live/dead stain Zombie NIR. EdU staining was performed using Click It Plus Alexa Flour 647 Assay kit (Invitrogen) according to manufacturer’s instructions. Samples were analyzed on a Fortessa LSR II (BD Biosciences). Data analysis was conducted using FlowJo v10 software (TreeStar). Gates for CD69, CD103 and PD-1 were set using fluorescence minus one samples. All antibodies were purchased from Biolegend. Relevant tetramers were kindly provided by Søren Buus, Department of Immunology and Microbiology and the NIH tetramer core facility.
Uddbäck I., Cartwright E.K., Schøller A.S., Wein A.N., Hayward S.L., Lobby J., Takamura S., Thomsen A.R., Kohlmeier J.E, & Christensen J.P. (2020). Long-term maintenance of lung resident memory T cells is mediated by persistent antigen. Mucosal immunology, 14(1), 92-99.
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2

Phenotypic Characterization of Antigen-Specific T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were first blocked for unspecific binding with αCD16/32 followed by staining with NP366–374/Db tetramer conjugated to allophycocyanin (APC) or Brilliant Violet 421 (BV421). Tetramer labelled cells was incubated with fluorophore conjugated antibodies CD8α (clone 53–6.7), CD8β (clone H35 17.2) CD44 (clone IM7), CD45.1 (clone 30-F11), CD45.2 (clone 104) CD69 (clone H1.2F3), CD103 (clone 2E7), PD-1 and live/dead stain Zombie NIR. EdU staining was performed using Click It Plus Alexa Flour 647 Assay kit (Invitrogen) according to manufacturer’s instructions. Samples were analyzed on a Fortessa LSR II (BD Biosciences). Data analysis was conducted using FlowJo v10 software (TreeStar). Gates for CD69, CD103 and PD-1 were set using fluorescence minus one samples. All antibodies were purchased from Biolegend. Relevant tetramers were kindly provided by Søren Buus, Department of Immunology and Microbiology and the NIH tetramer core facility.
Uddbäck I., Cartwright E.K., Schøller A.S., Wein A.N., Hayward S.L., Lobby J., Takamura S., Thomsen A.R., Kohlmeier J.E, & Christensen J.P. (2020). Long-term maintenance of lung resident memory T cells is mediated by persistent antigen. Mucosal immunology, 14(1), 92-99.
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