Biotinylated secondary antibodies for immunohistochemical assays

The following antibodies were used for immunofluorescent or immunohistochemical analysis: rabbit anti-Ki67 (VP-K451, 1:1000; Vector Laboratories; Burlingame, CA), rabbit anti-lysozyme (A0099, 1:1000; Dako; Glostrup Municipality, Denmark), mouse anti–E-cadherin (610181, 1:500; BD Transduction Laboratories; San Jose, CA), chicken anti-green fluorescent protein (GFP) for the detection of enhanced yellow fluorescent protein (EYFP) (GFP-1020, 1:2000; Aves Labs; Tigard, OR), rabbit anti-cyclin D1 (CRM 307, 1:100; Biocare Medical; Concord, CA), rabbit anti-cleaved caspase-3 (9661, 1:200; Cell Signaling Technology; Danvers, MA), mouse anti–β-catenin (610153, 1:200; BD Biosciences, San Jose, CA), and rabbit anti–mucin 2 (sc-15334, 1:1000; Santa Cruz Biotechnology; Dallas, TX). Cy2- and Cy3-conjugated fluorescent secondary antibodies were purchased from The Jackson Laboratory and used at a 1:200 dilution. Biotinylated secondary antibodies for immunohistochemical assays (Vector Laboratories) were used at a 1:200 dilution along with the ABC detection system (PK-6100; Vector Laboratories).