Westernbright sirius

Reagents were obtained from following manufacturers: Advansta Corporation (San Jose, USA): WesternBright Sirius®; Carl Roth GmbH & Co. KG (Karlsruhe, Germany): Carbenicillin disodium salt, lysozyme (≥ 45 000 FIP U/mg), ROTI®Fair Carbonate Bicarbonate Buffer pH 9.6, ROTI®Stock 10 × PBS, ROTI®Stock 10 × PBS-T, sodium chloride (≥ 99.5%), sodium dodecyl sulfate (SDS, ≥ 99.5%), sulfuric acid, Terrific-Broth- (TB-) Medium and urea (> 99.5%); GenScript Biotech BV (Leiden, Netherlands): SARS-CoV-2 Nucleocapsid protein (His-tag), SARS-CoV-2 Nucleocapsid Protein mAbs; MORPHISTO GmbH: Acetate buffer pH 5.0; Promega GmbH (Mannheim, Germany): Peroxidase-conjugated anti-human IgG antibody; Roche Deutschland Holding GmbH (Mannheim, Germany): cOmplete™ Mini EDTA-free protease inhibitor cocktail (from bovine pancreas); Seramun Diagnostika GmbH (Heidesee, Germany): TMB substrate solution; SERVA Electrophoresis GmbH (Heidelberg, Germany): Acrylamide/bis(acrylamide) (30% T, 2.67% C), BlueBlock PF 10x, Coomassie Brilliant Blue G-250, TEMED and trypsin (sequencing grade, MS approved); Sigma Aldrich Chemie GmbH (Taufkirchen, Germany): 2-mercaptoethanol (BioUltra), Antifoam Y-30 emulsion and imidazole (≥ 99.5%); Surmodics IVD, Inc. (Eden Prairie, USA): StabilZyme™ SELECT; Thermo Fisher Scientific (Waltham, Massachusetts, USA): AcroMetrix™ Inhibition Panel, SuperBlock^® (PBS).

Reagents were obtained from the following manufacturers: Advansta Corporation (San Jose, USA): WesternBright Sirius^®; Carl Roth GmbH & Co. KG (Karlsruhe, Germany): ROTI^®Stock 10× PBS, ROTI^®Stock 10× PBS-T, sodium chloride (≥99.5%), sodium dodecyl sulfate (SDS; ≥99.5%), and sulfuric acid; Promega GmbH (Mannheim, Germany): peroxidase-conjugated anti-human IgG Ab; GenScript (Leiden, Netherlands): SARS-CoV-2 Spike protein RBD (Omicron Variant, His Tag); Seramun Diagnostika GmbH (Heidesee, Germany): TMB substrate solution; SERVA Electrophoresis GmbH (Heidelberg, Germany): acrylamide/bis(acrylamide) (30% T, 2.67% C), BlueBlock PF 10×, Coomassie Brilliant Blue G-250, TEMED, and trypsin (sequencing grade, MS approved); Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany): ExtrAvidin-Peroxidase, imidazole (≥99.5%), 2-Mercaptoethanol (BioUltra), and polyethylenimin (PEI); Sino Biological (Eschborn, Germany): ACE-2 (His-tagged, biotinylated); Surmodics IVD, Inc. (Eden Prairie, USA): StabilZyme™ SELECT Assay diluent (Protein-free); Thermo Fisher Scientific (Waltham, MA, USA): goat anti-human IgA secondary Ab-HRP, Gibco DMEM, Gibco GlutaMAX Supplement, Gibco 100× MEM Non-Essential Amino Acids Solution, penicillin/streptomycin (10,000 U/mL), Gibco Fetal Bovine Serum (FBS), and SuperBlock^® (PBS).