Goat anti human cxcl13

Mouse pancreata were fixed and processed for histology and immunohistochemistry (IHC) as described previously(3 (link)). The IHC protocol was modified to detect mouse and human CXCL13, where blocking was done in 1× bovine free blocking solution (Vector) supplemented with 0.5% Tween-20, and 10% serum for 1 hour at room temperature, followed by incubation with the primary antibody diluted in 1× bovine free blocking solution overnight at 4°C. Secondary biotinylated rabbit-anti-goat antibody (Vector) was diluted in 1× bovine free blocking solution as well. The following primary antibodies were used: rabbit anti-GFP (#2956S, Cell Signaling), rat anti-B220 (#BDB557390, Fisher), rabbit-anti-vimentin (#5741P, Cell Signaling), mouse-anti-CD20 (#555677, BD Pharmingen), rabbit-anti-phospho Histone H3 (#06-570, Millipore), goat-anti-mouse CXCL13 and goat-anti-human CXCL13 (#AF470 and # AF801, both from R&D systems). At least 9 mice per experimental condition were analyzed for GFP staining and 6 mice per condition were analyzed for pHH3 staining. Slides were examined on a Nikon Eclipse 80i microscope.