Ab138513

Manufactured by Abcam

Sourced in United Kingdom

Ab138513 is a protein detection reagent. It is designed to bind and detect target proteins in various laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Cy3 conjugated affinipure donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) 510 meta, by Zeiss (1 mentions) Alexa fluor 594 donkey anti mouse igg h l, by Jackson ImmunoResearch (1 mentions) Opal polaris 7 color automation ihc detection kit, by Akoya Biosciences (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Bs 0549r, by Bioss Antibodies (1 mentions) Goat anti rabbit igg sc 2357, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase hrp conjugated goat anti mouse igg sc 516102, by Santa Cruz Biotechnology (1 mentions) Bs 0578r, by Bioss Antibodies (1 mentions) Modified masson s trichrome stain kit, by Solarbio (1 mentions) Mtt assay kit, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Anti ly6c, by Thermo Fisher Scientific (1 mentions) Anti pcna, by Santa Cruz Biotechnology (1 mentions) Anti ly6g, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Thermo Fisher Scientific (1 mentions) Ab9484, by Abcam (1 mentions) Pmsf protease inhibitor, by Thermo Fisher Scientific (1 mentions) Anti mouse or anti rabbit horseradish peroxidase conjugated antibodies, by Cell Signaling Technology (1 mentions) Nc membrane, by Merck Group (1 mentions)

5 protocols using ab138513

Histological and Immunofluorescence Analysis of Skin Samples

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Skin samples were fixed in 4% paraformaldehyde overnight at 4 °C, serially dehydrated and embedded in
paraffin. The specimens were sectioned and stained with hematoxylin and eosin (H&E). Immunofluorescence staining was performed
with routine antigen retrieval as suggested by the antibody manufacturer [41 (link)]. The slides were examined under a confocal microscope (Meta 510, Carl Zeiss). The primary antibodies for
immunostaining used in this study were as follows: fibrinogen beta-chain fragment (Abcam, EPR3083; 1:200), sulfated glycoprotein 1
(Abcam, ab68466; 1:200), galectin-1 (Abcam, ab138513; 1:200), lumican (Abcam, ab168348; 1:200), annexin A2 (Genetex, GTX101902,
1:200), gelsolin (Genetex, GTX101185; 1:200), apolipoprotein-A1 (Novus, NBP1-77008; 1:200) and fibronectin (Genetex, GTX61207;
1:200). Secondary antibodies were Alexa Fluor 594 donkey anti–mouse IgG (H+L) (Jackson ImmunoResearch,
715-585-151; 1:500), Cy3-conjugated AffiniPure donkey anti–rabbit IgG (Jackson ImmunoResearch, 711-165-152; 1:500) and
Alexa Fluor 594-AffiniPure F (ab′)2 Fragment rabbit anti–chicken IgY (IgG) (H+L) (Jackson ImmunoResearch,
303-586-003; 1:500). β-galactosidase activity and alkaline phosphatase activity were detected as described [43 (link),44 (link)].

Fan S.M., Tsai C.F., Yen C.M., Lin M.H., Wang W.H., Chan C.C., Chen C.L., Phua K.K., Pan S.H., Plikus M.V., Yu S.L., Chen Y.J, & Lin S.J. (2018). Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin. Biomaterials, 167, 121-131.

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Multiplex IHC Analysis of AML Bone Marrow

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Formalin-fixed bone marrow tissues from AML patients were processed through the routine IHC pipeline and stained for a rabbit anti-Galectin-1 (Abcam, ab138513). Multiplex IHC staining was performed with Akoya OpalTM seven-color fluorescent platform. Formalin-fixed, paraffin-embedded tissues were cut in 4-μm thick section and stained by Opal Polaris 7 Color Automation IHC Detection Kit (Akoya Biosciences, Menlo Park, CA) for simultaneous detection and quantitation of CD34 (GenTex, GTX28158), CD14 (Novusbio, NB100-2807), CD52 (Santa Cruz Biotechnology, sc-51560), SIGLEC10 (Sigma-Aldrich, HPA027093) and DAPI (Sigma-Aldrich). The slides were observed and imaged by Vectra Polaris automated quantitative pathology imaging system. The images were spectrally unmixed by Akoya phenoptics inForm software (inform 2.4.8).

Li K., Du Y., Cai Y., Liu W., Lv Y., Huang B., Zhang L., Wang Z., Liu P., Sun Q., Li N., Zhu M., Bosco B., Li L., Wu W., Wu L., Li J., Wang Q., Hong M, & Qian S. (2022). Single-cell analysis reveals the chemotherapy-induced cellular reprogramming and novel therapeutic targets in relapsed/refractory acute myeloid leukemia. Leukemia, 37(2), 308-325.

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Quantifying Extracellular Matrix Proteins

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The antibodies against galectin-1(ab138513),and GAPDH(ab9485) were from Abcam (Cambridge, UK). The polyclonal antibodies against collagen I (bs-0578R), collagen III (bs-0549R), and fibronectin 1 (FN1)/Ugl-Y3 (bs-13455R) were from Bioss (Beijing, China). The goat anti-rabbit IgG (sc-2357) and horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (sc-516102) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The MTT assay kit and dimethyl sulfoxide were from Sigma-Aldrich (St. Louis, MO, USA). The Modified Masson’s Trichrome Stain Kit was from Beijing Solarbio Science & Technology Co. Ltd. (G1346; Beijing, China).

Shen X., Liu H., Zhou H., Cheng Z., Liu G., Huang C., Dou R., Liu F, & You X. (2023). Galectin-1 promotes gastric cancer peritoneal metastasis through peritoneal fibrosis. BMC Cancer, 23, 559.

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Immunofluorescence Staining of Immune Cell Markers

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Tissue samples from ear or dorsal skin were embedded in OCT and frozen at −70 °C. Frozen tissue slides (20 μm) were fixed with 4% paraformaldehyde/PBS for 15 min followed by permeabilization and blocking with 0.2% Triton X-100, 10% FBS/PBS for 30 min at room temperature and stained with primary antibodies anti-Gal-7 (ab138513, Abcam), anti-CD11b (PA5-79532, ThermoFisher), anti-PCNA (sc-25280, Santa Cruz), anti-Gr1 (14-5931-81, eBioscience), Ly6C (128021, BioLegend) or Ly6G (127607, BioLegend) for 16 h at 4 °C. Slides were then washed in PBS and incubated with fluorochrome-conjugated secondary antibodies. To analyze Gal-7 binding to MDSCs, cells were differentiated from BM progenitors (as described below), activated with LPS (1 μg/ml; Invitrogen) for 16 h at 37 °C and incubated with rGal-7-PerCP/Cy5.5 (40 μg/ml), FITC-conjugated anti-Ly6C (green) and PE-conjugated anti-Ly6G (red) antibodies for 30 min. Cells were fixed with 4% paraformaldehyde/PBS for 15 min. Slides and cells were analyzed in an Olympus laser confocal microscope (Fluoview FV10i). At least 7 fields were analyzed for cell number and signal intensity.

Pinto N.A., Abba M.C., Laporte L., Pérez Sáez J.M., Blidner A.G., Torres N.I., Morales R.M., Gatto S.G., Bach C.A., Veigas F., García Rivello H.J., Song P., Frederiksen J.H., Rasmussen L.J., Poirier F., Croci D.O., Sundblad V., Rabinovich G.A, & Cerliani J.P. (2023). Galectin-7 reprograms skin carcinogenesis by fostering innate immune evasive programs. Cell Death and Differentiation, 30(4), 906-921.

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Galectin-1 Expression Analysis by Western Blot

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Cells were lysed in RIPA Lysis and Extraction Buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% P-40, 1% sodium deoxycholate, 0.1% SDS) with PMSF Protease Inhibitor (Thermo Fisher Scientific) added. Ten micrograms of purified proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on a 15% gradient gel (BioRad) and transferred onto NC membranes (EMD Millipore). Membranes were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-Galectin-1 (Abcam, ab138513, 1:1000), mouse anti-GAPDH (Abcam, ab9484, 1:5000). Anti-mouse or anti-rabbit horseradish peroxidase-conjugated antibodies (Cell Signaling Technology) were used as secondary antibodies.

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